Methylation status and genetic alterations of EGR1 in BC. (A) Methylation level of the EGR1 gene promoter in BC (TCGA Wanderer web tool). Median methylation level of the EGR1 gene promoter in BC. The box plot comparing specific CpG sites of EGR1 promoter methylation in normal (blue plot) and cancer tissue (red plot) was derived from the TCGA database (Methylation 450K) through the TCGA Wanderer web tool. The P values were obtained after an unpaired t-test using GraphPad Prism 7 software. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). (B) EGR1 mutation in human BC. Table showed the percentage of the mutation type of EGR1 in BC according to COSMIC database. (C) Alteration frequency of EGR1 mutation in BC was analyzed by using cBioPortal web. (D) Alteration frequency of EGR1 copy number in BC was analyzed by using cBioPortal web.