Analysis of EGR1 protein structure, post-translational modification, and expression in breast cancer (BC). (A) Schematic diagram of EGR1 protein structure and post-translational modifications. (B) mRNA expression of EGR1 in normal and BC tissue (IDBC, invasive ductal breast carcinoma) was derived from Oncomine database. (C) mRNA expression of EGR1 in breast normal and cancer tissues was derived from UALCAN web using TCGA database. (D) Protein expression of EGR1 in breast normal and cancer tissues by immunohistochemistry (IHC) was derived from Human Protein Atlas web. The intensity of EGR1 expression was quantified by ImageJ and plotted by GraphPad Prism 7 software (right panel). (E-G) mRNA expression of EGR1 in BC clinicopathological subtypes was analyzed using the BRCA TCGA datasets through UCSC Xena web. Box plots showing the EGR1 mRNA expression in BC subcategories including PAM50 subtypes (E), clinical subtypes (F), and stages (G). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.