Overview of DamID-derived technology, m6A-Tracer for labeling LADs in live-single cells during mitosis. (A) Schematic of the m6A-Tracer system. The DamID-derived method, m6A-Tracer is applied to visualize Lamin-associated domains (LADs), which are large regions of hetero-chromatin that interact with the lamina at the nuclear periphery. This method for imaging LADs utilizes Dam (dark brown) fused to Lamin B1 (brown). Dam deposits methyl groups at the N6 positions of adenine residue (m6A, red) embedded in 5’-GATC-3’ sites in DNA interacting with lamin B1. Catalytically inactivated DpnI (blue) fused with eGFP (green) called m6A-Tracer specifically binds to m6A, resulting in LADs marked by m6A-Tracer. (B) LAD dynamics during mitosis. Studies using m6A-Tracer technique have demonstrated stochastic positioning of LADs. During interphase, the mobility of LADs is confined to a narrow zone; but after mitosis, they are redistributed throughout the nucleus of the daughter cells. A new subset of LADs interacts with the nuclear lamina, and nucleoplasmic LADs can be in contact with nucleoli (gray).