Direct interaction between the LZ domain of βPIX and the tail domain of KIF5. (A) Schematic diagram of KIF5A constructs. (B, D) GST pulldown. Bound proteins were detected by immunoblotting with anti-His antibody (top). (C, E) Schematic diagram of the constructs for KIF5 tail (C) and βPIX (E). Each domain was expressed as His-tagged (C) or GFP-fused (E) protein. (F, G) Immunoprecipitation and immunoblotting. PC12 cells were transfected with plasmid encoding GFP-tagged βPIX construct (bottom) followed by immunoprecipitation with anti-GFP antibody. Bound KIF5A tail was detected by immunoblotting with anti-His antibody (top).