Kinetics of Kv7 subtype current inhibition facilitated by VSP activation. (A-C) Cells were cells transfected with Ciona intestinalis VSP (Ci-VSP) plus Kv7.2 (A), both Kv7.2 and Kv7.3 (B), or Kv7.3 alone (C) and depolarized to +30 mV for 42 s to deplete PI(4,5)P2 through Ci-VSP activation in the absence (black) or presence (blue) of EtOH. Relative current traces of the Kv7 channels subtracted to endogenous potassium current with Ci-VSP transfected are superimposed. (D-F) Time constants (τ) of Kv7 current inhibition by the activation of Ci-VSP in cells expressing Kv7.2 (n = 4 each) (D), both Kv7.2 and Kv7.3 (n = 6 for control, 7 for EtOH) (E), or Kv7.3 alone (n = 4 each) (F) without (black bars) and with (blue bars) 400 mM EtOH. Mann-Whitney u-test, *P < 0.05, **P < 0.01.