Non-stationary noise analysis (NSNA) for whole cell configuration of Kv7.2/7.3 channel with or without EtOH treatment. (A) Left, time-dependent regulation of Kv7.2/7.3 current upon treatment of 400 mM EtOH for 150 s. Right, summary of current inhibition by 400 mM EtOH (n = 9). (B) Representative traces of Kv7.2/7.3 current (upper panel) and variance (under panel) elicited in the absence (black) and presence (blue) of 400 mM EtOH in normal Ringer’s solution. (C) The variance/<I>max plotted against the open probability of Kv7.2/7.3 channel without (left) or with (right) 400 mM EtOH. Mean single channel current (i; D), channel number (N; E), and maxi-mal open probability (POmax; F) were calculated by non-stationary noise analysis (n = 9). Paired Student’s t-test were performed. ***P < 0.001.