Intracellular TEA and EtOH show differential effects on the activation and deactivation kinetics of Kv7.2/7.3 current in high-K+ external solution. Comparison of Kv7.2/7.3 current traces obtained from cells intracellular perfused with vehicle (control) or 400 mM EtOH (A) and 1 mM TEA (B). (C) Summary of time constants for de-activation and activation in cells perfused with 400 mM EtOH or 1 mM TEA. (control, n = 8; 400 mM EtOH, n = 8; 1 mM TEA, n = 4). One-way analysis of variance followed by Sidak’s posthoc test, **P < 0.01, as compared with control. n.s., non-significance. (D) De-activation kinetics of inward current at −70 mV after −20 mV (left) or +40 mV (right) pulse step in high-K+ external solution. Dotted line indicates Kv7.2/7.3 current in control cells. Dashed line is the zero-current level.