GSK5182 promotes osteoclast apoptosis. We incubated mature osteoclasts with GSK5182 (10 μM) or vehicle for 24 h. (A) The cells were fixed and subjected to TRAP staining. Apoptotic osteoclasts are marked by asterisks. (B) We measured osteoclast survival as the TRAP-positive MNC percentage. **P < 0.001. (C) We measured the osteoclast apoptosis rate by ELISA. **P < 0.001. (D) Total cell lysates were immunoblotted for cleaved caspase (cas)-3 and caspase-9. β-Actin served as a loading control. (E) A diagram showing the GSK5182 effect on osteoclast differentiation and apoptosis.