KLF15 regulates hepatic hepcidin gene expression and production in mouse primary hepatocytes. (A) Mouse primary hepatocytes were infected with Ad-GFP and Ad-Klf15 at a multiplicity of infection of 60 for 36 hr. Gene expressions were analyzed by qPCR using indicated primers. (B) Hepcidin concentrations in observed mouse primary hepatocytes. (C) Mouse primary hepatocytes were infected with Ad-Scram and Ad-shKlf15 at a multiplicity of infection of 60 for 36 hr and then treated with FSK for 12 hr. Total RNAs were utilized for qPCR analysis using gene-specific primers. (D) Hepcidin concentrations under indicated conditions. (E) WT mice were infected with Ad-Scram and Ad-shKlf15 for 7 days. These mice were then fasted for 24 hr. Quantitative PCR analysis was performed to determine Hamp expression in the liver. (F) Serum levels of hepcidin and iron under the observed condition. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. untreated controls, Ad-GFP, FSK-treated cells, fed, or fasted mice.