CD1d+ pMφ display tolerogenic phenotypes in CIA-induced CD1d KO hosts. CD1d KO mice were immunized by i.d. injections of 100 μg of chicken CII emulsified with an equal volume of CFA at the base of the tail to develop CIA. On day 21, mice were i.d. boosted with 100 μg of IFA. Seven days later, mice received i.v. injections of 1 × 106 TGF-β2-treated pMφ (Tol pMφ; ○), TGF-β2-untreated (pMφ; □), or PBS transfer as CIA control (◆). (A) Incidence and clinical scores of arthritis in groups received Tol pMφ; (B) Incidence and Clinical scores of arthritis in groups received pMφ. Each paw was scored from 0 to 5 according to the severity of arthritis, with a maximal cumulative score of 20. Results are representatives of three independent experiments. Data are presented as mean inflammatory scores (0-5) ± standard errors of the mean (SEM; 4-5 mice per group); *P < 0.05 (Student’s t-test).