CD1d+/− pMφ suppresses collagen-induced arthritis. (A) Experimental schedules. To induce CIA, CD1d+/− mice were immunized using intradermal tail base injections of 100 μg of chicken CII emulsified with an equal volume of CFA. Three weeks later, mice were i.d. boosted with 100 μg of CII in IFA. Seven days later, mice received intravenous injections of 1 × 106 CD1d−/− pMφ (○), CD1d+/− pMφ (□), or PBS as CIA control (◆). (B) Incidence of arthritic mice; (C) Clinical scores of arthritis in each group. Paws were scored from 0 to 5 according to the severity of arthritis, with a maximum cumulative score of 20. Results are presented as means ± SEM from three independent assessments (5 mice per group) of inflammation scores (0-5).