LPC exacerbated CHS in an IL17-dependent manner. (A) Ear thickness results after anti-IL17 mAb treatment. We pooled data; each circle represents a single mouse. (B, C) FACS analysis of the ear skin after anti-IL17 mAb treatment. The percentages of neutrophils (CD11b+ Ly6G+) in CD45+ population are shown in (B). (D) RT-qPCR analysis on the expressions of CXCL1 (left) and CXCL2 (right). We subjected the whole skin tissues to RNA extraction and used them for RT-qPCR study. Data are representative of two independent experiments. (E) FACS analysis on the expressions of IL17 and IFN-γ in the WT and G2A KO skins. The frequency of the cytokine-expressing cells in the CD45+ population is shown. Statistical analysis is shown in the right panel. Data are representative of two independent experiments and each circle represents one mouse. Data are presented as the mean ± SD. NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001.