LPC upregulated IL17. (A) The FACS analysis of IL17 and IFN-γ in CD45+ cells of the ears. Statistical analysis is shown in the right. We pooled data; each circle represents a single mouse. (B) RT-qPCR analysis on the expressions of IL17 and IFN-γ. We subjected the whole skin tissues to RNA extraction and used them for RT-qPCR study. Data are presented as the mean ± SD. NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001.