HDM-induced eosinophilic airway inflammation is suppressed by 5-/12-lipoxygenase blockade. (A, B) Mice were intraperitoneally injected with MK886 or baicalein 1 h before every challenge. We harvested lung and liver tissues 24 h after the last challenge, fixed them in formalin and embedded them in paraffin for section staining. (A) Lung tissue sections were stained with H&E (upper panel) or PAS (lower panel). We measured and scored peribronchial and perivascular lung inflammation or mucus secretion. Data are shown as the mean ± SD (n = 4-5 per group). *P < 0.05, ***P < 0.001 versus each control group. Scale bar, 100 µm. (B) We obtained total immune cells, eosinophils, neutrophils, monocytes, and lymphocytes in BALF using cytospin and stained them with Diff-quik. Data are shown as the mean ± SD (= 4-5 per group). ***P < 0.001 versus each control group. Scale bar, 50 µm.