HDM-induced TH2 cytokine synthesis is dependent on BLT1 and BLT2. (A-C) We randomly divided mice into four groups, gave them HDM sensitization and challenge, and sacrificed them 24 h after the last challenge. We collected BALF for ELISA. (A) Schematic of the HDM-induced eosinophilic airway inflammation model. We induced airway inflammation by sensitization with HDM (25 µg) and challenge with HDM (6.25 µg). We administered inhibitors via i.p. injection 1 h before every challenge. (B) We analyzed the levels of LTB4 and 12(S)-HETE in BALF using ELISA. Data are shown as the mean ± SD (n = 5-7 per group). ***P < 0.001 versus the control group. (C) We analyzed the levels of IL-4, IL-5, and IL-13 in BALF using ELISA. Data are shown as the mean ± SD (n = 7-12 per group). ***P < 0.001 versus the negative (NC) or positive (DMSO) control group.