Effect of myonectin on several pathways related to adipoge-nesis. (A) 3T3-L1 preadipocytes were induced to differentiate by the MDI cocktail with or without myonectin (1 μg/ml). Western blot analyses of p-AKT, p-AMPK and β-Catenin during the initial time of differentiation in the absence or presence of myonectin. Total AKT, total AMPK and HSP90 were presented as the controls for the normalization. (B-D) The ratios of p-AKT to total AKT, p-AMPK to total AMPK, and β-Catenin to HSP90. All quantitative data are the means ± SD (n = 3). *P < 0.05 compared to the myonectin-untreated con-trol cells (MDI).