Inhibitory function of myonectin involved in the early stage of adipogenesis. (A) Scheme for the preadipocyte differentiation and myonectin treatment. (B) Oil red O staining of lipid droplets after differentiation of the 3T3-L1 adipocytes treated with and without myonectin. (C) The levels of lipid accumulation of the 3T3-L1 mature adipocytes were quantified at 490 nm. (D) Protein levels and (E-G) mRNA levels of the adipogenic genes in mature 3T3-L1 adipocytes treated with and without myonectin. Each gene was normalized to Rpl32. All quantitative data are the means ± SD (n = 3-6). *P < 0.05, **P < 0.005, ***P < 0.0005 compared to the untreated control cells (Non) on day 10.