Down-regulated NDRG4 resulting from hypermethylation of DNA promoter. (A) NDRG4 mRNA expression was detected in PDAC (SW1990 and Canpan1) and HPNE cells treated with no drug, DAC, TSA, or DAC plus TSA. GAPDH was used as a loading control. The upper row shows qRT-PCR data and the lower row shows PCR bands. *P < 0.05, **P < 0.01, ns = no significance. (B) Hyper-methylation of the NDRG4 promoter was detected in SW1990, Canpan1, and HPNE cells by bisulfite-sequencing. Solid circles = methylated CpG site; Open circles = unmethylated CpG site.
