Down-regulated NDRG4 is closely associated with poor PDAC patient survival. (A) The P-value distribution of NDRG family members’ expression within PDAC patients’ prognosis was analyzed with Kaplan Meier plotter (http://kmplot.com/analysis/) and oncolnc (http://www.oncolnc.org/). (B) Kaplan-Meier plotter showed poor OS and less median survival time of PDAC patients with low NDRG4 expression (log-rank P = 0.035, HR = 0.64). (C) On-line analysis of Kaplan-Meier OS curves using TCGA dataset of PDAC patients by oncolnc. Patients were grouped on the basis of NDRG4 median expression value. (log-rank P = 0.0158). (D and E) NDGR4 expression levels were analyzed in tumors and adjacent tissues samples using two independent cohorts (D, GSE28735; E, GSE62452); Student's t-test, ***P < 0.001. (F) NDGR4 expression levels in PDAC cell lines and nonmalignant hTERT-HPNE (HPNE) cells were determined by immunoblotting.
