Hypo-CM derived from MSCs improved ischemic heart. (A) TUNEL-positive myocardial cells were significantly reduced in group treated with hypo-CM derived from MSCs, as compared to sham group (scale bar = 100 µm, magnification: 200×). (B) Fibrotic area confirmed by Masson’s trichrome staining was significantly decreased in treatment group of hypo-CM derived from MSCs (scale bar = 2 mm). (C) Quantitative analysis was performed to ascertain positive microvessel density. Stained cells were significantly increased in group exposed to hypo-CM derived from MSCs, as compared to control group (scale bar = 200 µm, magnification: 200×). Each experiment was repeated five times. Columns, mean; bars, SE. *P < 0.001, **P < 0.01.
