Effect of GSK-LSD1 on the inflammatory response in DSS-induced colitis. (A) Microscopic images of H&E stained colonic sections. Scale bars, 1 mm (upper panel) and 200 μm (lower panel). (B) Histological scores were measured based on the mucosal hyperemia, surface epithelium loss, and crypt damage. **P < 0.01. Each symbol represents an individual mouse from each group. (C) The amount of pro-inflammatory cytokines Il-1β, Il-6, and Il-8 was analyzed with RT-qPCR, 8 days after the start of the experiment. *P < 0.05 and **P < 0.01, (n = 3 per group). (D) Immunofluorescence staining of CD4, GR-1, and F4/80 in colon tissues. Nuclei were stained with DAPI. Scale bar = 100 μm. (E) Graphical overview of this study.
