Effect of GSK-LSD1 on alleviating DSS-induced colitis symptoms. (A) Schematic diagram of a DSS-induced colitis model. All groups were intraperitoneally injected with 5 mg/kg GSK-LSD1 or PBS, in equal volumes, on days 0, 1, and 3. (B) Body weight loss of mice was measured relative to initial weight. (C) The DAI of colitis was monitored daily. Statistical analysis used two-way ANOVA (PBS + DSS, n = 12; GSK-LSD1 + DSS, n = 11), *P < 0.05, ***P < 0.001. (D) Colon tissues of each group were obtained 8 days after the first DSS treatment. (E) Graph of colon length. *P < 0.05, ***P < 0.001. Each symbol represents an individual mouse from each group. (F) Immunofluorescence staining of Ki-67 in colon tissues. Nuclei were stained with DAPI. Scale bar = 100 μm.
