Analysis of histology and inflammatory response of Lsd1+/+ and Lsd1SA/SA mice in DSS-induced colitis. (A) Microscopic images of H&E stained colonic sections. Scale bars, 1 mm (upper panel) and 200 μm (lower panel). (B) Histological scores were determined based on the mucosal hyperemia, surface epithelium loss, and crypt damage. **P < 0.01. Each symbol represents an individual mouse from each group. (C) RT-qPCR data for the expression of pro-inflammatory Il-1β, Il-6, and Il-8 genes. (Lsd1+/+ and Lsd1SA/SA with water, n = 3; Lsd1+/+ with DSS, n = 5; and Lsd1SA/SA with DSS, n = 7), *P < 0.05, **P < 0.01. (D) Immunoblot analysis of iNOS in colons from Lsd1+/+ and Lsd1SA/SA mice. β-actin is an equal loading control. Each lane represents a mouse. (E) Immunofluorescence staining for CD4, GR-1, and F4/80 in colon tissues. Nuclei were stained with DAPI. Scale bar = 100 μm.
