Susceptibility analyses for DSS-induced colitis by comparing Lsd1+/+ and Lsd1SA/SA mice. (A) Schematic diagram of the DSS-induced colitis model. Male mice received 2% DSS in drinking water for 5 days, followed by normal drinking water for 5 days. (B) Body weight loss was measured relative to the initial weight (n = 17 per group). (C) The DAI of colitis was monitored daily (n = 17 per group). (D) Representative pictures of the colon. Colon tissue from each group was obtained 10 days after the first DSS treatment. (E) Graph of colon length. Each symbol represents an individual mouse from each group. (F) Immunofluorescence staining of Ki-67 in colon tissues. Nuclei were stained with DAPI. Scale bar = 100 μm. (G) Immunoblot analysis of PCNA in colons from Lsd1+/+ and Lsd1SA/SA mice. β-actin is an equal loading control. Each lane represents a mouse. **P < 0.01, ***P < 0.001 for whole figures.
