Comparison of conventional and real-time IVM. (A) Comparison of the frame rates of conventional IVM (non-video-rate, 1-2 fps) and real-time IVM (video-rate, 30 fps). Ear vessel of CX3CR1-GFP mice is shown. The white dotted ring indicates a flowing cell. Yellow camera icons indicate the timepoints for taking images. The flowing cell (B) and rolling cell (C) are tracked by real-time IVM. Ear vessel of CX3CR1-GFP mice is shown. The white dotted ring indicates the moving cells. Scale bar: 100 μm. RED, RITC-dextran 70.
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