Representative cartoon of the evolution of the directed mutation technique from enzyme restriction (A), homologous recombination (B), homing endonuclease (C), ZFN/TALEN (D) and the CRISPR/Cas9 system (E).

BMB Reports 2020;53:341-8 https://doi.org/10.5483/BMBRep.2020.53.7.070

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