Overview of next-generation sequencing techniques. (A) Detection methods for genetic variations, including in whole genome and whole exome. (B) Applications for methylation patterns. (C) Methods of genetic modifications, such as strand breaks enzyme digestion. (D) Various sequencing techniques for observing chromatin modifications. (E) Chromatin structures could be explained with sequencing applications. (F) Gene expression profiling with the form of RNAs. (G) Identification methods for RNA binding proteins and enrichment levels. (H) The formation of RNAs also detectable through sequencing applications.