Reduction in calpain-6 expression decreased levels of acetylated α-tubulin at lysine 40. Representative western blotting data in serum-starved NIH/3T3 cells. Starvation was maintained for 48 h. Experiments were performed three times independently. (A) Acetylated α-tubulin (K40) level decreased in capn6-deficient cells. AcTub (K40), acetylated α-tubulin at lysine 40. α-tub, α-tubulin. (B) Cells were treated with mixture of siCapn6 #1 and #2. αTat1 and Hdac6 protein levels were not affected upon capn6-knockdown.