Calpain-6 deficiency inhibited formation of primary cilia. (A) Calpain-6 protein levels were validated in starved NIH/3T3 cells. Capn6, calpain-6. (B) Primary cilia were observed in capn6-deficient NIH/3T3 cells. AcTub, acetylated α-tubulin (primary cilia). γ-Tub, γ-tubulin (centrosomes). Nuclei were counterstained with DAPI. Three independent experiments were performed. Bar = 5 μm. Graph presents the data as mean ± SD. *P < 0.05, and **P < 0.01 compared with the control cells. (C) Relative transcript levels of both Gli1 and Ptch1 were determined with and without SAG treatment in the serum-starved condition. Experiments were conducted more than three times independently. *P < 0.05 DMSO-treated cells were used as control. (D) Representative immunoblot data showing Gli1 protein levels.

BMB Reports 2019;52:619-24 https://doi.org/10.5483/BMBRep.2019.52.10.182

© 2019 BMB Reports