mRNA microarray to discover new regulators for ciliogenesis in NIH/3T3 cells. (A) NIH/3T3 cells were starved in a time-dependent manner. Ciliated cells were counted in randomly selected fields. Experiments were performed twice independently. AcTub, acetylated α-tubulin. Bar = 2 μm. Graph represents data as mean ± SD. **P < 0.01 compared with 0 h. (B) Strategy to discover novel regulators of ciliogenesis. (C) Heatmap showing all 62 increased genes. Fold change at each time point is presented as color.
