Efficacies of exosomal miRNA-584 derived from transfected MSCs for U87 cells. (A) Expression of exosomes markers were identified in exosomes isolated from CM of miRNA-584 transfected MSCs by western blot. (B) The qPCR data show miRNA-584 were high in U87 treated exosomes derived from transfected MSCs. (C) Caspase-3 activity was increased in co-cultured U87 with CM of transfected MSCs. (D, E) Proliferation assay and migration assay of U87 treated with exosomal miRNA-584 derived from transfected MSCs. Experiments were repeated three times. MSC-CM, culture media of MSCs; EV-584, extracellular vesicles (exosomal miRNA-584) derived from transfected MSCs. Columns, mean; bars, SE. (*P < 0.05).
