Effect of miRNA-584 for CYP2J2 expression and pathophysiological changes in miRNA-584 transfected U87 cells. (A) The data of RT-PCR show miRNA-584 transfection substantially reduced CYP2J2 expression of U87. (B) The sequence of miRNA-584 shows it regulates the expression of CYP2J2. (C) Transfection of miRNA-584 decreased CYP2J2 expression. (D) The data of luciferase assay indicate decreased CYP2J2 expression by miRNA-584 transfection. (E, F) Proliferation and migratory ability of miRNA-584 transfected U87 was analyzed with light microscopic photographs and statistical analysis. Experiments were repeated three times. LV-Scramble, lentivirus-pre-miRNA-scramble sequence; LV-miR-584, lentivirus-pre-miRNA-584. Columns, mean; bars, SE. (*P < 0.05).
