Effect of Med on lipid droplet morphology in BAT cells. (A) Treatment procedure of Med in BAT cells. (B) Viability of BAT cells was assessed by the MTT assay. Data are expressed as mean ± SEM of three independent experiments. PA means preadipocyte and Med 5 μM, 10 μM and 20 μM concentrations are used. PA was used as control and *P ≤ 0.05. (C) TEM images (3500× magnification) of Med treated BAT cells (Scale bar represents 5 μm). Red arrows indicate lipid droplets (LD). (D) Relative LD diameter was measured by manual measurement of diameter of 5 biggest LDs from each samples and then calculating their averages.
