Transduction efficiency of PEP-1-FK506BP. 0.5–5 μM of PEP-1-FK506BP proteins was added to WT9-7 cell culture media for 2 h (A) or PEP-1-FK506BP (5 μM) proteins was added to the cell culture media for 10–120 min (B). The stability of PEP-1-FK506BP. WT9-7 cells were treated with 5 μM PEP-1-FK506BP and incubated for 1–60 h, analyzed by Western blotting, and band intensity was measured by densitometry (C). WT9-7 cells were treated with PEP-1-FK506BP (5 μM) and rapamycin (0.5 nM) for 24 h. Cell proliferation was determined (D). *P < 0.05 and **P < 0.01, compared with control cells.