Purification and transduction of PEP-1-FK506BP into WT9-7 cells. Purified PEP-1-FK506BP and control FK506BP were analyzed by 15% SDS-PAGE (A) and subjected to Western blot analysis with an anti-histidine antibody (B). Transduction of PEP-1-FK506BP into WT9-7 cells. The cells were treated with PEP-1-FK506BP (5 μM) proteins for 2 h and observed by confocal microscopy (C). Scale bar = 20 μm.
