NGBR knockdown abrogates the VEGF-induced endothelial migration. (A) Cell migration assays after VEGF (50 ng/ml) treatment of HUVEC cultures transfected with NgBR siRNA or negative control siRNA. (B) NgBR mRNA expression in HUVEC cultures after NgBR knockdown. (C) NgBR protein expression in HUVECs after NgBR knockdown. **p < 0.001, ***p < 0.0001 vs. controls, calculated by unpaired two-tailed Student’s t-tests. Error bars, standard error of the mean.