• Impact of Mesenchymal Stem Cell Senescence on Inflammaging

    MSCs as a key modulator of inflammaging. The diagram illustrates how senescence and inflammation regulate MSC fate and lead to inflammaging. Cellular senescence facilitates MSCs to differentiate toward adipogenesis, and biased adipogenesis in the BM niche skews hematopoietic reconstitution, inhibits lymphopoiesis but increased myelopoiesis. Aged MSCs show increased secretion of extracellular vesicles (miR-146a) and SASP that stimulates innate immune cell receptors or macrophage polarization from M2 to M1. Age-related alterations may contribute to increased MDS or AML-related mutations or 2-HG production that possibly further exacerbated hematopoietic niche function or the pathogenesis of myeloid malignancies. BM, bone marrow; EV, extracellular vesicle; MDS, myelodysplastic syndrome; AML, Acute myeloid leukemia; 2-HG, 2-hydroxyglutarate; SASP, Senescence-associated secretory phenotype.
  • Traction Force Microscopy for Understanding Cellular Mechanotransduction

    Traction force microscopy (TFM)-based cell-ECM force quantification. (A) Schematic diagram for typical TFM platform using deformable substrates, where fluorescence beads (orange dots) are embedded. Cells can adhere to the substrate through surface-conjugated ECMs or protein ligands (purple line). Traction forces (indicated by red arrows) exerted by cells can cause subtle deformation of a substrate, where traction forces can be measured by tracking the displacement of fluorescent beads within the substrate. (B) Traction force stress map showing human bone marrow-derived mesenchymal stem cells adhered onto hydrophobic-polydimethylsiloxane (PDMS) and hydrophilic-PDMS with polyethyleneoxide (PEO) (PEO-PDMS), with varying stiffness ranging from 0.2-0.3 kPa (soft, 70:1) to 5-6 kPa (intermediate, 60:1). (C, D) Schematic and scanning electron microscopy (SEM) image of 2D TFM by micropillars. Vertical arrays of PDMS microposts are fabricated by a photolithography technique. Cell spreads across multiple post beds on which ECMs are pre-coated. Adhered cells can exert traction forces. Traction forces are calculated from the deflection and material property (spring constant) of microposts. (E, F) Schematic representations of traditional 2D TFM method (E) and novel 3D TFM method (F). 3D TFM determines both horizontal (dh) and vertical (dv) components of the displacement vector (d), allowing the calculation of a 3D traction force vector. (G) A breast tumor cell (yellow, MDA-MB-231 cell line) is embedded in 3D type I collagen matrix, visualized by reflective confocal images (cyan). (H) 3D rendering images of bead displacements (blue) and cells (magenta) in 3D collagen matrix. *Figures adapted with permission from; Fig. 1A, B: ref. (32, Fig. 1C, D: ref. (36, Fig. 1E, F: ref. (40, Fig. 1G, H: ref. (44).

BMB Reports 2020; 53(2): 65~123
Invited Mini Reviews
Impact of Mesenchymal Stem Cell Senescence on Inflammaging
Byung-Chul Lee & Kyung-Rok Yu
BMB Reports 2020; 53(2): 65-73  https://doi.org/10.5483/BMBRep.2020.53.2.291
Traction Force Microscopy for Understanding Cellular Mechanotransduction

Sung Sik Hur, Ji Hoon Jeong, Myung Jin Ban, Jae Hong Park, Jeong Kyo Yoon & Yongsung Hwang

BMB Reports 2020; 53(2): 74-081  https://doi.org/10.5483/BMBRep.2020.53.2.308
Articles
Circular RNA hsa_circ_0075828 Promotes Bladder Cancer Cell Proliferation Through Activation of CREB1
Chengle Zhuang, Xinbo Huang, Jing Yu & Yaoting Gui
BMB Reports 2020; 53(2): 82-87  https://doi.org/10.5483/BMBRep.2020.53.2.059
Overcoming Multidrug Resistance by Activating Unfolded Protein Response of the Endoplasmic Reticulum in Cisplatin-Resistant A2780/CisR Ovarian Cancer Cells
Euitaek Jung, Dongsoo Koh, Yoongho Lim, Soon Young Shin & Young Han Lee
BMB Reports 2020; 53(2): 88-93  https://doi.org/10.5483/BMBRep.2020.53.2.108
Functional Analysis of RNA Motifs Essential for BC200 RNA-mediated Translational Regulation
Seonghui Jang, Heegwon Shin & Younghoon Lee
BMB Reports 2020; 53(2): 94-99  https://doi.org/10.5483/BMBRep.2020.53.2.153
Heterogeneity in Liver Histopathology Is Associated With GSK-3β Activity and Mitochondrial Dysfunction in End-Stage Diabetic Rats on Differential Diets
Jun-Ho Lee, Soo-Bong Choi, Dong-Jun Sung, Mingli Jin, Ju-Han Lee, Ji-Young Mun, Tae-Sook Hwang, Sang-Don Han, Young-Tae Ro, Sung-Young Kim, Jueng-Soo You, Inja Lim & Yun-Hee Noh
BMB Reports 2020; 53(2): 100-105  https://doi.org/10.5483/BMBRep.2020.53.2.114
PEP-1-GLRX1 Protein Exhibits Anti-Inflammatory Effects by Inhibiting the Activation of MAPK and NF-κB Pathways in Raw 264.7 Cells
Min Jea Shin, Dae Won Kim, Yeon Joo Choi, Hyun Ju Cha, Sung Ho Lee, Sunghou Lee, Jinseu Park, Kyu Hyung Han, Won Sik Eum & Soo Young Choi
BMB Reports 2020; 53(2): 106-111  https://doi.org/10.5483/BMBRep.2020.53.2.180
Methylated-UHRF1 and PARP1 Interaction Is Critical for Homologous Recombination
Ja Young Hahm, Joo-Young Kang, Jin Woo Park, Hyeonsoo Jung & Sang-Beom Seo
BMB Reports 2020; 53(2): 112-117  https://doi.org/10.5483/BMBRep.2020.53.2.264
Characterization of Human Cardiac Mesenchymal Stromal Cells and Their Extracellular Vesicles Comparing With Human Bone Marrow Derived Mesenchymal Stem Cells
In Sook Kang, Joowon Suh, Mi-Ni Lee, Chaeyoung Lee, Jing Jin, Changjin Lee, Young Il Yang, Yangsoo Jang & Goo Taeg Oh
BMB Reports 2020; 53(2): 118-123  https://doi.org/10.5483/BMBRep.2020.53.2.235

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February 2020
Volume 53
Issue 2

2018 SCI Impact Factor 2.966

BIOCHEMISTRY & MOLECULAR BIOLOGY 134/292

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