• Evolution of CRISPR towards accurate and efficient mammal genome engineering

    Small molecules enhance knock-in efficiency. (A) Small molecules related to the NHEJ or HDR repair pathway. Inhibitors are labeled in red, activators are labeled in blue. NU7026 inhibits DNA-PK, and SCR7, E1B55K, and E4orf6 inhibit DNA ligase IV. MLN4924, NSC15520, RS-1, Trichostatin A, or Resveratrol enhance CtIP, RPA, RAD51, or ATM, respectively. ATM protein also induces activation of RPA, BRCA2, and RAD51. The i53 is an inhibitor of 53BP1. The i53 activates DNA end resection and recruitment, of BRCA1 to DSBs. (B) HDR activity is increased at S/G2 phase. NHEJ activity is labeled in blue, HDR activity is labeled in red. Small molecules are used to arrest the cell cycle at specific phase, to improve HDR efficiency. L755505, Resveratrol, Mimosine, Aphidicolin, Thymidine and Hydroxyurea block cells at the G1 to S phase before DNA replication, and Nocodazole arrests cell cycle at G2/M phase. Lovastatin also inhibits at early G1, and partially at G2/M phase.
  • Stem cells and reproduction

    Current applicable assisted reproductive technologies for mitochondrial replacement. From an MII oocyte with mutated mtDNA, spindle or PB1 is isolated and transferred to an enucleated MII oocyte with healthy mtDNA. Reconstructed oocytes by ST or PB1T are fertilized with sperm. In the zygote with mutated mtDNA, PNs or PB2 can be transferred to enucleated zygotes with healthy mtDNA. Two PNs are enucleated for PNT while only one PN is for PB2T. For SCNT, the fibroblasts carrying mutant mtDNA are transferred to enucleated MII oocytes with healthy mtDNA. Reconstructed SCNT oocytes are activated artificially. Embryos with healthy mtDNA can develop into ESCs or offspring. MII, metaphase II; mtDNA, mitochondrial DNA; ST, spindle transfer; PB1T, 1st polar body transfer; PB2T, 2nd polar body transfer; PNT, pronucleus transfer; SCNT, somatic cell nuclear transfer; ESCs, embryonic stem cells.

BMB Reports 2019; 52(8): 475~531
Invited Mini Reviews
Evolution of CRISPR towards accurate and efficient mammal genome engineering
Seuk-Min Ryu, Junseok W Hur & Kyoungmi Kim
BMB Reports 2019; 52(8): 475-481  https://doi.org/10.5483/BMBRep.2019.52.8.149
Stem cells and reproduction
Yeonmi Lee & Eunju Kang*
BMB Reports 2019; 52(8): 482-489  https://doi.org/10.5483/BMBRep.2019.52.8.141
Mycoplasma exploits mammalian tunneling nanotubes for cell-to-cell dissemination
Bong-Woo Kim, Jae-Seon Lee & Young-Gyu Ko
BMB Reports 2019; 52(8): 490-495  https://doi.org/10.5483/BMBRep.2019.52.8.243
A chemical conjugate between HER2-targeting antibody fragment and Pseudomonas exotoxin A fragment demonstrates cytotoxic effects on HER2-expressing breast cancer cells
Sunju Lee, Sangsu Park, Minh Tan Nguyen, Eunyoung Lee, Julee Kim, Sangki Baek, Chong Jai Kim, Yeon Jin Jang & Han Choe
BMB Reports 2019; 52(8): 496-501  https://doi.org/10.5483/BMBRep.2019.52.8.250
JNK activation induced by ribotoxic stress is initiated from 80S monosomes but not polysomes
Tae-Sung Kim, Hag Dong Kim, Yong Jun Park, EunBin Kong, Hee Woong Yang, Youjin Jung, YongJoong Kim & Joon Kim
BMB Reports 2019; 52(8): 502-507  https://doi.org/10.5483/BMBRep.2019.52.8.273
An alpha-lipoic acid-decursinol hybrid compound attenuates lipopolysaccharide-mediated inflammation in BV2 and RAW264.7 cells
Mi-Youn Kwon, Jiwon Park, Sang-Min Kim, Jooweon Lee, Hyeongjin Cho, Jeong-Ho Park & Inn-Oc Han
BMB Reports 2019; 52(8): 508-513  https://doi.org/10.5483/BMBRep.2019.52.8.144
Flrt2 is involved in fine-tuning of osteoclast multinucleation
Jumpei Shirakawa, Noriko Takegahara, Hyunsoo Kim, Seoung Hoon Lee, Kohji Sato, Satoru Yamagishi & Yongwon Choi
BMB Reports 2019; 52(8): 514-519  https://doi.org/10.5483/BMBRep.2019.52.8.116
Dihydroartemisinin inhibits HepG2.2.15 proliferation by inducing cellular senescence and autophagy
Jiang Zou, Qiang Ma, Ru Sun, Jiajing Cai, Hebin Liao, Lei Xu, Jingruo Xia, Guangcheng Huang, Lihua Yao, Yan Cai, Xiaowu Zhong & Xiaolan Guo
BMB Reports 2019; 52(8): 520-525  https://doi.org/10.5483/BMBRep.2019.52.8.058
Akap12beta supports asymmetric heart development via modulating the Kupffer’s vesicle formation in zebrafish
Jeong-gyun Kim, Hyun-Ho Kim & Sung-Jin Bae
BMB Reports 2019; 52(8): 526-531  https://doi.org/10.5483/BMBRep.2019.52.8.111


Current Issue

August 2019
Volume 52
Issue 8

2018 SCI Impact Factor 2.966


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