• LRRK2 and membrane trafficking: nexus of Parkinson's disease

    Overview of LRRK2 and its substrates in vesicle or membrane trafficking. (Top) Schematic representation of LRRK2 domains, including armadillo repeats (ARM), ankyrin repeats (ANK), leucine-rich repeats (LRR), a ROC domain, a COR domain, a kinase domain, and a C-terminal WD40 domain. Pathogenic mutations are indicated above. (Bottom) Two categories of LRRK2 substrates discussed in this review. LRRK2 phosphorylates a subset of Rab GTPases, master regulators of vesicle trafficking (left), and key molecules of synaptic transmission (right).

  • Deep sequencing of B cell receptor repertoire

    Experimental workflow of repertoire sequencing. (A) Sorted B cells by cell surface markers are prepared for sequencing in bulk or single cell state with further isolation by droplet microfluidics. (B) Templates available for BCR repertoire analysis are both gDNA and cDNA. gDNA contains intron sequences and both V and J genes that are not taken part in V(D)J recombination, resulting in far genomic distance between V region and C region in particular B cell clones. In contrast, only rearranged V(D)J segments exist in cDNA. They are juxtaposed with the C region. (C) For both gDNA and cDNA, a library is constructed by using PCR with multiplex primers targeting multiple V segments. Alternatively, in order to prevent primer bias from a large number of primer sets, a universal forward priming site is attached to the 5′ RACE region by template switching. (D) Once library preparation is completed, NGS platform is chosen considering the depth and length of BCR to be examined.


BMB Reports 2019; 52(9): 533~576
Invited Mini Reviews
LRRK2 and membrane trafficking: nexus of Parkinson's disease
Eun-Mi Hur, Eun-Hae Jang, Ga Ram Jeong & Byoung Dae Lee
BMB Reports 2019; 52(9): 533-539  https://doi.org/10.5483/BMBRep.2019.52.9.186
Deep sequencing of B cell receptor repertoire
Daeun Kim & Daechan Park*
BMB Reports 2019; 52(9): 540-547  https://doi.org/10.5483/BMBRep.2019.52.9.192
Articles
Ets-1 enhances tumor migration through regulation of CCR7 expression
Li-Wen Fang, Ying-Hsien Kao, Ya-Ting Chuang, Huey-Lan Huang & Tzong-Shyuan Tai
BMB Reports 2019; 52(9): 548-553  https://doi.org/10.5483/BMBRep.2019.52.9.232
Tissue-resident natural killer cells exacerbate tubulointerstitial fibrosis by activating transglutaminase 2 and syndecan-4 in a model of aristolochic acid-induced nephropathy
Yu Mee Wee, Heounjeong Go, Monica Young Choi, Hey Rim Jung, Yong Mee Cho, Young Hoon Kim, Duck Jong Han & Sung Shin
BMB Reports 2019; 52(9): 554-559  https://doi.org/10.5483/BMBRep.2019.52.9.193
6-sialyllactose ameliorates dihydrotestosterone-induced benign prostatic hyperplasia through suppressing VEGF-mediated angiogenesis
Eun-Yeong Kim, Bo-Ram Jin, Tae-Wook Chung, Sung-Jin Bae, Hyerin Park, Dongryeol Ryu, Ling Jin, Hyo-Jin An & Ki-Tae Ha
BMB Reports 2019; 52(9): 560-565  https://doi.org/10.5483/BMBRep.2019.52.9.113
ACOX1 destabilizes p73 to suppress intrinsic apoptosis pathway and regulates sensitivity to doxorubicin in lymphoma cells
Fei-Meng Zheng, Wang-Bing Chen, Tao Qin, Li-Na Lv, Bi Feng, Yan-Ling Lu, Zuo-Quan Li, Xiao-Chao Wang, Li-Ju Tao, Hong-Wen Li & Shu-You Li
BMB Reports 2019; 52(9): 566-571  https://doi.org/10.5483/BMBRep.2019.52.9.094
miR-101-3p/Rap1b signal pathway plays a key role in osteoclast differentiation after treatment with bisphosphonates
Jie Li, You Li, Shengjie Wang, Hui Che, Jun Wu & Yongxin Ren
BMB Reports 2019; 52(9): 572-576  https://doi.org/10.5483/BMBRep.2019.52.9.076

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September 2019
Volume 52
Issue 9

2018 SCI Impact Factor 2.966

BIOCHEMISTRY & MOLECULAR BIOLOGY 134/292

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