• Techniques for investigating mitochondrial gene expression

    An overview of biochemical and imaging tools for investigating mitochondrial gene expression. Mitochondrial DNA (A) mt-ZFN and mt-TALEN use a zinc-finger DNA-binding domain and transcription activator-like elements, respectively, and each links to a DNA-cleavage domain of the restriction enzyme, Fok1. (B) EtBr or SyBr binds to double-stranded mt-DNA, whereas BrdU and EdU can incorporate into mt-DNA during mt-DNA replication, which allows intact mt-DNA to be visualized in live cells. (C) An mt-DNA specific sequence can be detected by using a FISH probe. (D) GFP-tagged TFAM is also used to visualize mt-DNA in live cells. Mitochondrial RNA (E) Three possible mechanisms of RNA transport into mitochondrial matrix are suggested: (1) PNPASE transports RNA of RNase P, RNAs of mitochondrial ribosomal proteins, and 5S rRNAs by recognizing the stem-loop structure of their RNAs; (2) miRNA can be delivered to mitochondria by Ago2; (3) PNPASE also translocates pre-miRNA into mitochondria. (F) RNA recognition motif domains of human PUMILIO1 are tagged with split fragments of EGFP. When the two split EGFPs are reassembled by binding to ND6 mRNA, EGFP signals appear and the dynamics of ND6 mRNA can be visualized. Mitochondrial protein (G) Mitochondria-specific metabolic labeling can be achieved by using isotope or non-canonical amino acids while inhibiting cytoplasmic translation. Isotope-labeled mitochondrial proteins are separated by SDS-PAGE. Azide-conjugated non-canonical amino acids are detected by biotin or a fluorescent dye through click reaction.
  • Peripheral inflammatory biomarkers in Alzheimer’s disease: a brief review

    Graphical summary of peripheral inflammatory biomarker candidates.
  • Translocator protein (TSPO): the new story of the old protein in neuroinflammation

    Sequence alignment of eukaryotic TSPO homologues. (A) TSPO has a highly conserved sequence, especially on transmembrane domains from plants to human. (B) TSPO topology in the mitochondrial membrane.
  • Sphingolipids in neuroinflammation: a potential target for diagnosis and therapy

    Sphingolipid metabolism and interconnected bioactive sphingolipid metabolites. The various enzymes that mediate interconversion between various bioactive lipids are localized with the lipids themselves. Ceramide is produced from sphingomyelin by SMase. Ceramide can be hydrolyzed to sphingosine, which is then phosphorylated to sphingosine-1-phosphate (S1P) by sphingosine kinase (SphK).

BMB Reports 2020; 53(1): 1~63
Editorial
Mitochondria and neurodegenerative diseases: Special issue of BMB Reports in 2020
Kwang Chul Chung
BMB Reports 2020; 53(1): 1-2  https://doi.org/10.5483/BMBRep.2020.53.1.310
Invited Mini Reviews
Techniques for investigating mitochondrial gene expression
Dongkeun Park, Soyeon Lee & Kyung-Tai Min
BMB Reports 2020; 53(1): 3-9  https://doi.org/10.5483/BMBRep.2020.53.1.272
Peripheral inflammatory biomarkers in Alzheimer’s disease: a brief review
Jong-Chan Park, Sun-Ho Han & Inhee Mook-Jung*
BMB Reports 2020; 53(1): 10-19  https://doi.org/10.5483/BMBRep.2020.53.1.309
Translocator protein (TSPO): the new story of the old protein in neuroinflammation
Younghwan Lee, Youngjin Park, Hyeri Nam, Ji-Won Lee & Seong-Woon Yu
BMB Reports 2020; 53(1): 20-27  https://doi.org/10.5483/BMBRep.2020.53.1.273
Sphingolipids in neuroinflammation: a potential target for diagnosis and therapy
Ju Youn Lee, Hee Kyung Jin & Jae-sung Bae
BMB Reports 2020; 53(1): 28-34  https://doi.org/10.5483/BMBRep.2020.53.1.278
Emerging perspectives on mitochondrial dysfunction and inflammation in Alzheimer’s disease
Seung-Min Yoo, Jisu Park, Seo-Hyun Kim & Yong-Keun Jung*
BMB Reports 2020; 53(1): 35-46  https://doi.org/10.5483/BMBRep.2020.53.1.274
Mitochondrial dysfunction and Alzheimer's disease: prospects for therapeutic intervention
Ji Woong Lim, Jiyoun Lee & Ae Nim Pae
BMB Reports 2020; 53(1): 47-55  https://doi.org/10.5483/BMBRep.2020.53.1.279
The central regulator p62 between ubiquitin proteasome system and autophagy and its role in the mitophagy and Parkinson's disease
Woo Hyun Shin, Joon Hyung Park & Kwang Chul Chung*
BMB Reports 2020; 53(1): 56-63  https://doi.org/10.5483/BMBRep.2020.53.1.283

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January 2020
Volume 53
Issue 1

2018 SCI Impact Factor 2.966

BIOCHEMISTRY & MOLECULAR BIOLOGY 134/292

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