BMB Rep. 2016; 49(6): 331-336  
Induction of tolerance against the arthritogenic antigen with type-II collagen peptide-linked soluble MHC class II molecules
Yoon-Kyung Park, Sundo Jung & Se-Ho Park*
Department of Life Sciences, Korea University, Seoul 02841, Korea
Correspondence to: Tel: +82-2-3290-3160; Fax: +82-2-927-9028; E-mail: sehopark@korea.ac.kr
Received: October 13, 2015; Revised: November 3, 2015; Accepted: January 10, 2016; Published online: June 30, 2016.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Abstract
In murine collagen-induced arthritis (CIA), self-reactive T cells can recognize peptide antigens derived from type-II collagen (CII). Activation of T cells is an important mediator of autoimmune diseases. Thus, T cells have become a focal point of study to treat autoimmune diseases. In this study, we evaluated the efficacy of recombinant MHC class II molecules in the regulation of antigen-specific T cells by using a self peptide derived from CII (CII260-274; IAGFKGEQGPKGEPG) linked to mouse I-Aq in a murine CIA model. We found that recombinant I-Aq/CII260-274 molecules could be recognized by CII-specific T cells and inhibit the same T cells in vitro. Furthermore, the development of CIA in mice was successfully prevented by in vivo injection of recombinant I-Aq/CII260-274 molecules. Thus, treatment with recombinant soluble MHC class II molecules in complex with an immunodominant self-peptide might offer a potential therapeutic for chronic inflammation in autoimmune disease such as rheumatoid arthritis.
Keywords: Collagen-induced arthritis, Immunotherapy, Rheumatoid arthriti, Recombinant MHC II, Type-II collagen

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