BMB Rep. 2016; 49(7): 388-393  
Methyltransferase and demethylase profiling studies during brown adipocyte differentiation
Min Jeong Son1,#, Won Kon Kim1,2,#, Kyoung-Jin Oh1, Anna Park1, Da Som Lee1, Baek Soo Han1,2, Sang Chul Lee1,2,* & Kwang-Hee Bae1,2,*
1Metabolic Regulation Research Center, KRIBB, 2Department of Functional Genomics, University of Science and Technology (UST), Daejeon 34141, Korea
Correspondence to: Kwang-Hee Bae, Tel: +82-42-860-4268; Fax: +82-42-860-4269; E-mail: khbae@kribb.re.kr, Sang Chul Lee, Tel: +82-42-860-4145; Fax: +82-42-860-4593; E-mail: lesach@kribb.re.kr #These two authors contributed equally.
Received: March 22, 2016; Revised: March 30, 2016; Accepted: May 2, 2016; Published online: July 31, 2016.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Abstract
Although brown adipose tissue is important with regard to energy balance, the molecular mechanism of brown adipocyte differentiation has not been extensively studied. Specifically, regulation factors at the level of protein modification are largely unknown. In this study, we examine the changes in the expression level of enzymes which are involved in protein lysine methylation during brown adipocyte differentiation. Several enzymes, in this case SUV420H2, PRDM9, MLL3 and JHDM1D, were found to be up-regulated. On the other hand, Set7/9 was significantly down-regulated. In the case of SUV420H2, the expression level increased sharply during brown adipocyte differentiation, whereas the expression of SUV420H2 was marginally enhanced during the white adipocyte differentiation. The knock-down of SUV420H2 caused the suppression of brown adipocyte differentiation, as compared to a scrambled control. These results suggest that SUV420H2, a methyltransferase, is involved in brown adipocyte differentiation, and that the methylation of protein lysine is important in brown adipocyte differentiation.
Keywords: Brown adipocytes, Demethylase, Methyltransferase, Obesity, SUV420H2


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