BMB Rep. 2016; 49(4): 232-237  
Anti-inflammatory action of ethanolic extract of Ramulus mori on the BLT2-linked cascade
Geun-Soo Park1, Jeong-Keun Kim2 & Jae-Hong Kim1,*
1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, 2Department of Chemical Engineering and Biotechnology, Korea Polytechnic University, Shihung 15073, Korea
Correspondence to: Tel: +82-2-3290-3452; Fax: +82-2-3290-3938; E-mail: jhongkim@korea.ac.kr
Received: January 6, 2016; Revised: January 13, 2016; Accepted: February 16, 2016; Published online: April 30, 2016.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Abstract
Mulberry tree twigs (Ramulus mori) contain large amounts of oxyresveratrols and have traditionally been used as herbal medicines because of their anti-inflammatory properties. However, the signaling mechanism by which R. mori exerts its anti-inflammatory action remains to be elucidated. In this study, we observed that R. mori ethanol extracts (RME) exerted an inhibitory effect on the lipopolysaccharide (LPS)-induced production of the pro-inflammatory cytokine interleukin-6 (IL-6) in Raw264.7 macrophage cells. Additionally, RME inhibited IL-6 production by blocking the leukotriene B4 receptor- 2 (BLT2)-dependent-NADPH oxidase 1 (NOX1)-reactive oxygen species (ROS) cascade, leading to anti-inflammatory activity. Finally, RME suppressed the production of the BLT2 ligands LTB4 and 12(S)-HETE by inhibiting the p38 kinase- cytosolic phospholipase A2-5-/12-lipoxygenase cascade in LPS-stimulated Raw264.7 cells. Overall, our results suggest that RME inhibits the ‘BLT2 ligand-BLT2’-linked autocrine inflammatory axis, and that this BLT2-linked cascade is one of the targets of the anti-inflammatory action of R. mori.
Keywords: BLT2, IL-6, Ramulus mori, Raw264.7 cells, ROS


This Article

e-submission

Archives