BMB Rep. 2016; 49(4): 201-207  
Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
Seung Hwan Lee1 & Sangsu Bae2,3,*
1Center for Genome Engineering, Institute for Basic Science, Seoul 08826, 2Department of Chemistry, 3Institute for Materials Design, Hanyang University, Seoul 04763, Korea
Correspondence to: Tel: +82-2-2220-2618; Fax: +82-2-2298-0319; E-mail: sangsubae@hanyang.ac.kr
Received: February 29, 2016; Published online: April 30, 2016.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Abstract
The CRISPR-Cas system has emerged as a fascinating and important genome editing tool. It is now widely used in biology, biotechnology, and biomedical research in both academic and industrial settings. To improve the specificity and efficiency of Cas nucleases and to extend the applications of these systems for other areas of research, an understanding of their precise working mechanisms is crucial. In this review, we summarize current studies on the molecular structures and dynamic functions of type I and type II Cas nucleases, with a focus on target DNA searching and cleavage processes as revealed by single- molecule observations.
Keywords: CRISPR-Cas system, Crystal structure, Single molecule, Type I CRISPR, Type II CRISPR


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