BMB Rep. 2016; 49(4): 197-198  
An efficient SCNT technology for the establishment of personalized and public human pluripotent stem cell banks
Jeoung Eun Lee1, Young Gie Chung2, Jin Hee Eum1, Yumie Lee2 & Dong Ryul Lee1,2,3,*
1CHA Stem Cell Institute, CHA University, Seongnam 13488, Korea, 2Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA, 3Department of Biomedical Science, CHA University, Seongnam 13488, Korea
Correspondence to: E-mail:
Received: March 14, 2016; Published online: April 30, 2016.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Although three different research groups have reported successful derivations of human somatic cell nuclear transfer-derived embryonic stem cell (SCNT-ESC) lines using fetal, neonatal and adult fibroblasts, the extremely poor development of cloned embryos has hindered its potential applications in regenerative medicine. Recently, however, our group discovered that the severe methylation of lysine 9 in Histone H3 in a human somatic cell genome was a major SCNT reprogramming barrier, and the overexpression of KDM4A, a H3K9me3 demethylase, significantly improved the blastocyst formation of SCNT embryos. In particular, by applying this new approach, we were able to produce multiple SCNT-ES cell lines using oocytes obtained from donors whose eggs previously failed to develop to the blastocyst stage. Moreover, the success rate was closer to 25%, which is comparable to that of IVF embryos, so that our new human SCNT method seems to be a practical approach to establishing a pluripotent stem cell bank for the general public as well as for individual patients.
Keywords: ESC derivation, HLA-matched, Pluripotent stem cells, Somatic cell nuclear transfer, Stem cell bank, Therapeutic cloning

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