BMB Rep. 2015; 48(12): 655-667  
Adult stem cell lineage tracing and deep tissue imaging
Juergen Fink#, Amanda Andersson-Rolf# & Bon-Kyoung Koo*
Department of Genetics and Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, CB2 1QR, United Kingdom
Correspondence to: E-mail: bkk25@cam.ac.uk
Received: December 1, 2015; Published online: December 31, 2015.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Abstract
Lineage tracing is a widely used method for understanding cellular dynamics in multicellular organisms during processes such as development, adult tissue maintenance, injury repair and tumorigenesis. Advances in tracing or tracking methods, from light microscopy-based live cell tracking to fluorescent label- tracing with two-photon microscopy, together with emerging tissue clearing strategies and intravital imaging approaches have enabled scientists to decipher adult stem and progenitor cell properties in various tissues and in a wide variety of biological processes. Although technical advances have enabled time-controlled genetic labeling and simultaneous live imaging, a number of obstacles still need to be overcome. In this review, we aim to provide an in-depth description of the traditional use of lineage tracing as well as current strategies and upcoming new methods of labeling and imaging.
Keywords: Adult stem cells, Advanced imaging, Intravital imaging, Lineage tracing, Tissue clearing


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