BMB Rep. 2014; 47(2): 110-114  
Comparative evaluation of the biological properties of fibrin for bone regeneration
Joung-Hwan Oh1, Hye-Jin Kim1, Tae-Il Kim2 & Kyung Mi Woo1,3,*
1Department of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, 2Department of Periodontology, School of Dentistry, Seoul National University, 3Department of Dental Pharmacology & Therapeutics, School of Dentistry, Seoul National University, Seoul 110-749, Korea
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Received: July 4, 2013; Revised: June 6, 2013; Accepted: July 9, 2013; Published online: February 28, 2014.
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Fibrin is a natural provisional matrix found in wound healing, while type I collagen is a major organic component of bone matrix. Despite the frequent use of fibrin and type I collagen in bone regenerative approaches, their comparative efficacies have not yet been evaluated. In the present study, we compared the effects of fibrin and collagen on the proliferation and differentiation of osteoblasts and protein adsorption. Compared to collagen, fibrin adsorbed approximately 6.7 times more serum fibronectin. Moreover, fibrin allowed the proliferation of larger MC3T3-E1 pre-osteoblasts, especially at a low cell density. Fibrin promoted osteoblast differentiation at higher levels than collagen, as confirmed by Runx2 expression and transcriptional activity, alkaline phosphatase activity, and calcium deposition. The results of the present study suggest that fibrin is superior to collagen in the support of bone regeneration.
Keywords: Collagen, Fibrin, Fibronectin, Osteoblast, Protein adsorption

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