BMB Rep. 2014; 47(2): 92-97  
Sequence-specific interaction between ABD-B homeodomain and castor gene in Drosophila
Keon-Hee Kim & Siuk Yoo*
Department of Life Sciences, College of Sciences, Yeungnam University, Gyeongsan 712-749, Korea
Correspondence to: Tel: +82-53-810-2378; Fax: +82-53-810-4618; E-mail: yoosiuk@ynu.ac.kr
Received: May 22, 2013; Revised: June 6, 2013; Accepted: June 12, 2013; Published online: February 28, 2014.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Abstract
We have examined the effect of bithorax complex genes on the expression of castor gene. During the embryonic stages 12-15, both Ultrabithorax and abdominal-A regulated the castor gene expression negatively, whereas Abdominal-B showed a positive correlation with the castor gene expression according to real-time PCR. To investigate whether ABD-B protein directly interacts with the castor gene, electrophoretic mobility shift assays were performed using the recombinant ABD-B homeodomain and oligonucleotides, which are located within the region 10 kb upstream of the castor gene. The results show that ABD-B protein directly binds to the castor gene specifically. ABD-B binds more strongly to oligonucleotides containing two 5'-TTAT-3' canonical core motifs than the probe containing the 5'-TTAC-3' motif. In addition, the sequences flanking the core motif are also involved in the protein-DNA interaction. The results demonstrate the importance of HD for direct binding to target sequences to regulate the expression level of the target genes.
Keywords: Abdominal-B, Castor, EMSA, Homeodomain, Homeotic gene


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