BMB Reports 2018; 51(7): 362-367  https://doi.org/10.5483/BMBRep.2018.51.7.101
Protective effects of Tat-DJ-1 protein against streptozotocin-induced diabetes in a mice model
Hyeon Ji Yeo1,#, Eun Ji Yeo1,#, Min Jea Shin1,#, Yeon Joo Choi1, Chi Hern Lee1, Hyeok Yil Kwon2, Dae Won Kim3, Won Sik Eum1,* & Soo Young Choi1,*
1Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 24252, 2Department of Physiology, College of Medicine, Hallym University, Chuncheon 24252, 3Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, Korea
Correspondence to: Soo Young Choi, Tel: +82-33-248-2112; Fax: +82-33-248-3202; E-mail: sychoi@hallym.ac.kr; Won Sik Eum, Tel: +82-33-248-3221; Fax: +82-33-248-3202; E-mail: wseum@hallym.ac.kr
#These authors contributed equally to this work.
Received: May 2, 2018; Revised: May 21, 2018; Accepted: June 1, 2018; Published online: July 31, 2018.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

cc This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
A major feature of type 1 diabetes mellitus (T1DM) is hyperglycemia and dysfunction of pancreatic β-cells. In a previous study, we have shown that Tat-DJ-1 protein inhibits pancreatic RINm5F β-cell death caused by oxidative stress. In this study, we examined effects of Tat-DJ-1 protein on streptozotocin (STZ)-induced diabetic mice. Wild type (WT) Tat-DJ-1 protein transduced into pancreas where it markedly inhibited pancreatic β-cell destruction and regulated levels of serum parameters including insulin, alkaline phosphatase (ALP), and free fatty acid (FFA) secretion. In addition, transduced WT Tat-DJ-1 protein significantly inhibited the activation of NF-κB and MAPK (ERK and p38) expression as well as expression of COX-2 and iNOS in STZ exposed pancreas. In contrast, treatment with C106A mutant Tat-DJ-1 protein showed no protective effects. Collectively, our results indicate that WT Tat-DJ-1 protein can significantly ameliorate pancreatic tissues in STZ-induced diabetes in mice.
Keywords: Blood glucose, Diabetes mellitus, Insulin, Protein therapy, Tat-DJ-1


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Funding Information
  • National Research Foundation of Korea(NRF)
      10.13039/501100003725
      2014R1A1A4A01008026, 2016R1D1A3B03932554, NRF-2009-0093812
  • Ministry of Education(Ministry of Education of the Republic of Korea)
      10.13039/501100002701
     

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