BMB Reports 2017; 50(4): 175-185
Nonsense-mediated mRNA decay at the crossroads of many cellular pathways
Fabrice Lejeune
1University Lille, UMR8161 – M3T – Mechanisms of Tumorigenesis and Target Therapies, France, 2CNRS, UMR 8161, France, 3Institut Pasteur de Lille, F-59000 Lille, France
Correspondence to: Tel: +33-320871059; Fax: +33-320871111; E-mail:
Received: January 19, 2017; Published online: April 30, 2017.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.


Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism ensuring the fast decay of mRNAs harboring a premature termination codon (PTC). As a quality control mechanism, NMD distinguishes PTCs from normal termination codons in order to degrade PTC-carrying mRNAs only. For this, NMD is connected to various other cell processes which regulate or activate it under specific cell conditions or in response to mutations, mis-regulations, stresses, or particular cell programs. These cell processes and their connections with NMD are the focus of this review, which aims both to illustrate the complexity of the NMD mechanism and its regulation and to highlight the cellular consequences of NMD inhibition.

Keywords: Cellular pathway, Nonsense mutations, Nonsense-mediated mRNA decay (NMD), Quality control mechanism, UPF proteins

Nonsense-mediated mRNA decay (NMD) is a surveillance or quality control mechanism which targets for accelerated decay mRNAs harboring a premature termination codon (PTC) (17). In mammals, NMD is activated on an mRNA when the first stop codon of the open reading frame is located more than 50–55 nucleotides upstream of an exon-exon junction (8). Recent studies have shown that NMD can also be elicited when the distance between the first stop codon of the open reading frame and the poly(A) binding protein becomes abnormally long (912). From these two NMD activation models has emerged a combinatory model taking both originally proposed activation pathways into account, but this model requires further experimental support (6, 11, 13).

The proteins UPF1, UPF2, and UPF3X (also called UPF3b) play a central role in NMD and are universally conserved within the eukaryotic kingdom (14). Extensive studies on UPF1 have demonstrated that this phosphorylated protein is an RNA helicase that clears the region downstream from a PTC to prepare the mRNA for decay (1520). Phosphorylation and subsequent dephosphorylation of UPF1 are required to elicit NMD. In addition to their role in NMD, UPF proteins are involved in other cellular mechanisms (see below). Since NMD can be mechanistically different according to the species (14), this review focuses mainly on NMD in human cells and on interactions between the NMD mechanism or NMD factors and other processes occurring in human cells. In particular, we discuss the connections between NMD and upstream or downstream processes involved in gene expression and the influence of various cell pathways on NMD.


The EJC-dependent activation model

Experimental data show that a stop codon is recognized as a PTC only if it is located more than 50–55 nucleotides upstream of an exon-exon junction (Fig. 1A) (2123). A protein complex called EJC (for Exon Junction Complex) is deposited 20–24 nucleotides upstream of exon-exon junctions after pre-mRNA splicing occurs (24, 25). EJC recruits NMD factors UPF3X/UPF3b and then UPF2. During the pioneer round of translation when the 5′-cap is still bound by the CBP80/CBP20 heterodimer and the poly(A) tail is bound by the poly(A) binding proteins N1 and C1 (PABPN1 and PABPC1, respectively), the first ribosome reads the mRNA and removes all EJCs present on its path (26). If the mRNA carries no stop codon more than 50–55 nucleotides upstream of an exon-exon junction, no EJCs will remain on the mRNA once the ribosome reaches the normal stop codon after the pioneer round of translation. The mRNP will then be remodeled to undergo steady-state translation with replacement of CBP80/CBP20 by eIF4E and the presence of PABPC1 only on the poly(A) tail. If the mRNA carries a stop codon located more than 50–55 nucleotides upstream of an exon-exon junction, the ribosome will pause at this stop codon. At least one EJC will still be present on the mRNA when the ribosome reaches this stop codon. This EJC then recruits the SURF complex formed by SMG1, SMG8, SMG9, UPF1, and the release factors 1 and 3 (16). SMG1 phosphorylates UPF1, causing the departure of the ribosome and the release factors (20). Then SMG5, SMG6, SMG7, and the protein phosphatase 2A (PP2A) are recruited, causing the exit of SMG1, SMG8, and SMG9. Phosphorylated UPF1 then interacts directly with the RNA, and its RNA helicase activity is activated so as to remove proteins from the region downstream from the PTC on the mRNA and to prepare the mRNA for decay (15). The RNA helicase MOV10 also seems to be involved in the removing of RNA secondary structures and proteins downstream of PTCs (27). Phosphorylated UPF1 also interacts with the proline-rich nuclear receptor coregulatory protein 2 (PNRC2) to recruit the decapping enzyme DCP1a, involved in the 5′ to 3′ decay pathway (28). Then PP2A dephosphorylates UPF1, and the mRNA decay pathways are activated to degrade the mRNA, from both ends and also via endonucleolytic cleavage by SMG6 in the vicinity of the PTC (2932).

In this model, NMD occurs only during the pioneer round of translation. This round is characterized by a dedicated translation initiation complex and the presence of the heterodimer CBP80/CBP20 on the 5′cap, of EJCs on the mRNA, and of PABPC1 and PABPN1 on the poly(A) tail (27, 33, 34). According to the model, only newly synthesized mRNAs are targeted for NMD, and if they are not recognized as NMD targets during the pioneer round of translation, they will be immune to NMD (27, 35).

The EJC-dependent model fits most cases, but some experimental data challenge this model. For instance, EJCs are not present upstream of all exon-exon junctions (36, 37). Furthermore, some PTCs located less than 50 nucleotides upstream of the last exon-exon junction trigger NMD (38, 39), and some PTCs located more than 55 nucleotides upstream of the last exon-exon junction do not (40, 41).

PTC-PABPC1 distance-dependent activation

The second model describing activation of NMD in mammalian cells is based on the distance between the PTC and the PABPC1. This model is similar to the model of NMD activation in other species such as yeast, worm, and fly (14). Basically, when the 3′UTR of an mRNA is abnormally long because of the presence of a PTC for instance, NMD is activated to degrade that specific mRNA (42). In the above-mentioned species, the size of the 3′UTR tends to be relatively homogenous, which facilitates recognition of abnormally long 3′UTRs (43). In mammals, the molecular mechanism may be more complex because of evolution, and particularly because of the highly variable size of the 3′UTR, from 21 nucleotides to more than 8 kb (44).

Many reports indicate that the distance between PABPC1 and the PTC is what triggers NMD (912, 45). This model suggests that certain cis- or trans-acting elements can provide a measure of the length of the 3′UTR. In particular, it is thought that specific elements might be involved in regulating NMD on mRNAs with long 3′UTRs and in preventing unnecessary degradation by NMD. Among these regulatory elements, secondary structures in the 3′UTR might bring PABPC1 closer to the normal stop codon and thus prevent inappropriate activation of NMD under specific conditions (10). Long human 3′UTRs have also been reported to contain NMD-inhibiting regulatory sequences (46).

In the PTC-PABPC1 distance model, NMD occurs during all cycles of translation in an EJC-independent way (47, 48). UPF1 has been shown to interact directly with RNA and to concentrate in the 3′UTR (4952). UPF1 and PABPC1 thus compete to terminate the translation by interacting with release factor 3 when the ribosome reaches a stop codon that will trigger NMD (in the case of UPF1) or the normal translation termination (in the case of PABPC1) (Fig. 1B). The closer the PTC to the start of the open reading frame, the greater the chances that UPF1 will be involved in terminating translation and eliciting NMD. This model thus suggests a gradient of NMD efficiency, decreasing with increasing proximity of the PTC to the physiological stop codon (38).

The combinatory model

Given the strong experimental evidence supporting each of these two models, it is difficult to ignore one of them. A combinatory model has been proposed to reconcile the two visions, taking both proposed pathways of NMD activation into account (6, 8, 13). In this model, a PTC can be recognized at two successive steps. During the pioneer round of translation, the EJC-dependent NMD activation pathway would be responsible for recognition of PTC-containing mRNAs as described above. Later, as a fraction of the PTC-containing mRNAs can escape NMD (for example, by being protected from translation for a while), the PTC-PABPC1 distance pathway would take charge of these PTC-containing mRNAs during later rounds of translation. Although such a model can explain the vast majority of cases of NMD activation, some exceptions have already been described that do not fit with this model, indicating that NMD activation remains incompletely understood (5355).


In mammals, splicing has been implicated in NMD since the discovery that at least one intron must be present downstream of a PTC on the pre-mRNA in order to elicit NMD on the corresponding mRNA (23, 56, 57). These are the studies that gave rise to the “rule of the 50–55 nucleotides” (41), stating that a PTC elicits NMD only if an exon-exon junction is present more than 50–55 nucleotides downstream of the PTC (58). The molecular explanation came with the identification of a protein complex deposited 20–24 nucleotides upstream of exon-exon junctions as a consequence of splicing (24, 25). This complex, called EJC for Exon Junction Complex, is formed by splicing factors (RNPS1, SRm160, UAP56, SAP18, Acinus, Pnn/DRS, and Pinin), exporting factors (UAP56, REF/Aly, TAP/p15, Y14, or Magoh), and a group of four proteins forming the core of the EJC (Y14, Magoh, eIF4AIII, and MLN51) (5971). This complex is fully exported to the cytoplasm before undergoing the pioneer round of translation responsible for PTC detection, in keeping with the cytoplasmic cellular location of NMD (45, 71).

While splicing is required for NMD, NMD is also very useful in eliminating defective or unwanted mRNA products generated by splicing. Splicing is indeed the major source of PTC-containing mRNAs, since one-third of the mRNAs generated by alternative splicing are targeted for NMD because of the presence of a PTC (72, 73). As more than 95% of multi-exon pre-mRNAs are subject to alternative splicing (74), NMD needs to be very efficient to eliminate all the unwanted splicing products. Alternative splicing and NMD are involved in regulating many genes under specific cellular conditions. For example, splicing factors such as members of the SR family (75) decrease their own synthesis, when their level is too high, by incorporating into their mRNAs, via alternative splicing, a poison cassette exon harboring an NMD-eliciting PTC (76, 77).


MicroRNAs (miRNAs) regulate gene expression by either inducing the decay or inhibiting the translation of their target mRNAs. NMD and the miRNA pathway interact at two different levels at least. Firstly, miR128 is reported to regulate the expression of both the UPF1 gene and the MLN51 gene, encoding one of the core components of the EJC (78). This finding suggests fine regulation of NMD efficiency by the miRNA pathway, although the specific conditions under which miR128 targets UPF1 and/or MLN51 mRNAs remain to be elucidated. Secondly, a factor playing a role in both the NMD and miRNA decay pathways has been identified. mRNA decay via the miRNA pathway involves proteins, such as Argonaute, found also in the siRNA pathway. This makes Argonaute a key player of two RNA decay pathways. The protein Argonaute 2 is also reported to inhibit the pioneer round of translation by competing with the heterodimer CBP80/CBP20 for binding to the 5′ cap (79, 80). Since CBP80 plays a role in NMD by promoting the interaction between UPF1 and UPF2 during the pioneer round of translation, binding of Argonaute 2 to the 5′ cap promotes NMD inhibition (7981). It is worth noting that this competition between CBP80/20 and Argonaute 2 occurs only on mRNAs carrying a binding site for miRNA in the 3′UTR. This limits the number of mRNAs subject to this regulation.


Staufen 1 and Staufen 2 are RNA-binding proteins targeting for accelerated decay mRNAs containing a specific type of hairpin or a double-stranded structure made of complementary Alu elements in the 3′UTR. Staufen proteins can recruit UPF1 to the mRNA independently of other UPF proteins or EJC components. Once UPF1 is recruited, mRNA decay is triggered by a machinery that has not been yet identified (29). SMD and NMD share a central protein, UPF1, and thus compete for the available UPF1. An example of this competition concerns the proteins myogenin, required for myogenesis, and PAX3, a transcription factor that inhibits the differentiation of myoblasts to myotubes (82). Myogenin mRNA is a natural NMD target and PAX3 mRNA is an SMD substrate. UPF1 can interact either with UPF2, to induce NMD, or with Staufen proteins, to trigger SMD. If the UPF1-UPF2 interaction is favored, NMD will be active, myogenin mRNA will be degraded, and myogenesis will be inhibited. If the UPF1-Staufen protein interaction is favored, SMD will be active, PAX3 mRNA will be degraded, and this will lead to the differentiation of myoblasts to myotubes (83).

NMD factors have been implicated in many different mechanisms that could be antagonistically linked to NMD according to the same principle of factor availability as described above. For instance, as UPF1 has been shown to play a role in maintaining telomere length, in S-phase progression, and in histone mRNA decay (84, 85). All these processes could be antagonistically related to NMD.


NMD and translation are tightly interconnected at different levels. First, PTC recognition requires the translation machinery. Interference with translation by chemicals, suppressor tRNAs, or secondary structures blocking ribosome progression has been shown to impair NMD (86, 87). In particular, the translation inhibitors cycloheximide, puromycine, and emetin are reported to strongly inhibit NMD (87, 88). Second, NMD affects mRNAs being translated during both the pioneer round and other rounds of translation (26, 47, 48). During the pioneer round, a peptide is synthesized (89), consistently with the presence of CBP80-bound mRNAs in the subpolysomal fractions of a polysome gradient (33), but this peptide is targeted for rapid decay via stimulation of the proteasome by UPF1 (90, 91). Third, several translation initiation factors are required for NMD, such as eIF4G (34), eIF2α (33), eIF4F (47), and eIF3e (92, 93). Interestingly, these factors are tightly regulated. An example is eIF2α, whose phosphorylated isoform is inactive. Its phosphorylation is induced by various cell stresses, such as hypoxia, endoplasmic reticulum stress due to accumulation of unfolded proteins, and amino acid starvation leading to inhibition of the pioneer round of translation and hence to NMD (33, 94, 95). Fourth, NMD activation, or more precisely UPF1 phosphorylation, leads to inhibition of translation, so as to prevent initiation of new translation cycles and the synthesis of truncated proteins (96). Finally, the NMD factors UPF1, UPF2, UPF3/UPF3a, and UPF3X/UPF3b have been shown to stimulate translation when tethered to an unspliced mRNA (97, 98). Although its molecular mechanism remains unclear, this translation stimulation illustrates the close interconnection of NMD and translation.


Readthrough is a natural process leading to incorporation of an amino acid into the growing peptide when the ribosome reaches a stop codon during translation (6, 99). So far, readthrough has been found to regulate the expression of four genes in human cells (100). Molecules such as aminoglycosides, ataluren/PTC124, and readthrough compounds (RTC) induce readthrough of PTCs but not of physiological stop codons (99, 101, 102). The involvement of UPF proteins in this mechanism is controversial. Knockout of UPF proteins in yeast was first found to increase readthrough, and this was attributed to decreased translation termination fidelity (103, 104). Then it was proposed that readthrough efficiency is independent of the presence or absence of UPF proteins (105). In human cells, knockdown of UPF proteins has recently been reported to impair readthrough. This suggests a role of these proteins in readthrough in humans and hence a link between NMD and readthrough (106).

Favoring PTC readthrough is a highly promising therapeutic approach for genetic diseases caused by a nonsense mutation (107). Yet the readthrough efficiency generated even in the presence of readthrough activator molecules remains low, likely because PTC-readthrough substrates are first NMD substrates, so that the amount of PTC-containing mRNAs available for readthrough is very low. It has therefore been proposed to combine NMD inhibitors with readthrough activators, to improve the synthesis of full-length readthrough proteins. Such combinations have been shown to increase substantially the production of the readthrough proteins from PTC-containing mRNAs in both cell cultures (108, 109) and in vivo, in a mouse model (110). Yet the fact that UPF proteins are required for both PTC readthrough and NMD suggests that strategies for inhibiting NMD should exclude targeting any UPF protein, as this would compromise the efficiency of readthrough. The role of UPF proteins in readthrough should be studied in depth in order to propose an efficient therapeutic approach to treating nonsense-mutation-related diseases.


In mammalian cells, NMD has been clearly demonstrated to be a cytoplasmic event (45) occurring during or soon after mRNA export to the cytoplasm (111). Furthermore, NMD factors and substrates have been reported to transit through cytoplasmic foci called P-bodies (112, 113). P-bodies are foci scattered through the cytoplasm and containing RNA-degrading enzymes and RNAs such as miRNAs and PTC-containing mRNAs (114, 115). As PTC-containing mRNAs are exported to the cytoplasm and transit through P-bodies before being degraded, this suggests that they are transported from the nucleus to P-bodies at least. It has recently been reported that to promote this transport and allow NMD, an active cytoskeleton is required (106). Interestingly, when the cytoskeleton is impaired and in particular when the microtubules are disrupted, NMD factors and substrates are found concentrated in P-bodies, suggesting that the cytoskeleton is responsible either for transporting to P-bodies a factor required for NMD or for transporting PTC-containing mRNPs from P-bodies to the cellular location of NMD (106).


When cells undergo apoptosis, a specific gene expression profile is activated so as to induce cell death. This profile includes both the transcription of specific genes and the decay of targeted proteins. Investigators have wondered whether a quality control mechanism such as NMD is still active during apoptosis or whether it becomes useless, since the accumulation of nonfunctional or deleterious truncated proteins could even be part of the cell death process. This question was recently answered in two studies where the efficiency of NMD was measured after activation of apoptosis with chemicals (116, 117). Both studies demonstrated that NMD is inhibited during apoptosis. The molecular mechanism was also determined: UPF1 and UPF2 are targeted and inactivated by caspases 3 and 7. In addition, the UPF fragments generated by caspase cleavage show apoptotic activity, inducing an amplification loop that precipitates cell death (116). This amplification loop is also generated upon NMD inhibition, via expression of apoptotic genes which are normally repressed by NMD: genes encoding cell cycle inhibitors and the caspase activators GADD45α, GADD45β, and CDKN1A (117).

The findings of both studies show that an RNA surveillance mechanism such as NMD can be regulated according to the cell status and is a direct target during programmed cell death by apoptosis.


NMD is a quality control mechanism and a gene regulation pathway for about 5–10% of human genes (118). When NMD is impaired, many mRNAs are up-regulated and can be translated. The mRNA encoding ATF4 is a natural substrate of NMD which becomes up-regulated when NMD is inhibited. ATF4 is a transcription factor activating the transcription of genes involved in autophagy, a process through which both proteins and organelles are degraded (119). Inhibition of NMD by means of an siRNA against UPF1 or UPF2 causes activation of ATF4 expression, promoting increased autophagy in the cell in response to the synthesis of aberrant proteins. Conversely, activation of NMD by overexpression of UPF1, for instance, leads to minimal autophagy in the cell (120). The ultimate consequence of autophagy is cell death. Autophagy is thus an additional programmed cell death pathway related to NMD inhibition (116, 117), alongside apoptosis.


About 30% of proteins present in a cell are secreted or transmembrane proteins. They are thus synthesized and folded in the endoplasmic reticulum (ER). When unfolded or misfolded proteins accumulate in the ER, a protein degradation pathway, called the unfolded protein response (UPR), is activated to eliminate them. This response involves activation of certain transcription factors and inhibition of translation, which stops the synthesis of unfolded or misfolded proteins. Another consequence of UPR activation is inhibition of NMD (121). NMD, as a process regulating the expression of some genes, influences the expression of several genes involved in UPR, such as ATF3, ATF4, ATF6, and IREα. Inhibition of NMD thus creates a positive feedback loop (122124). Interestingly, inhibition of NMD as a first event does not induce UPR. This indicates that the origin of this regulation loop must be ER stress, and not NMD inhibition unrelated to ER stress (122). The ER could also modulate the efficiency of NMD in another way. The ER is a calcium storage site that can regulate the cytoplasmic concentration of calcium (125). Recently, an increase in the intracellular calcium concentration was found to inhibit NMD (126). This opens prospects for a new therapeutic approach to inhibiting NMD in some cases of nonsense-mutation-related genetic diseases.


As described above, many genes involved in splicing or NMD (among others) use NMD to regulate their own expression. This suggests that no NMD at all would probably promote chaos in the cell. Accordingly, knockout of UPF1, UPF2, or SMG1 in mouse leads to embryo death very early during development (127129). This suggests a major role of NMD during development and particularly in the expression of genes involved in the early development and differentiation program. In support of the view that NMD plays a key role in development and in the homeostasis of multi-cellular organisms, only a few patients with a default in the NMD machinery have been reported. Some cases of mental retardation have been attributed to loss of UPF2, UPF3, UPF3X, SMG6, or of an EJC component such as eIF4A3 or RNPS1 (130136).

In mice where UPF2 was conditionally knocked out, some cell populations, such as proliferative cells, were found to be more sensitive than others to a lack of NMD (137). In hematopoietic cells, genomic DNA rearrangements at particular loci generate specific isoforms of immunoglobulins or T cell receptor (TCR). These DNA rearrangements attest the wide diversity of peptides involved in antigen recognition. Yet two-thirds of these DNA rearrangements result in a faulty message with a PTC and are silenced thanks to NMD (138). When NMD is deficient, aberrant shortened peptides are synthesized from the PTC-containing TCR or immunoglobulin mRNAs and interfere with the lymphocyte maturation process, causing developmental arrest and death of the organism. This highlights the crucial roles played by NMD (139).


Besides its interconnection with endogenous cell processes, NMD can also interact with exogenous machineries. For instance, viruses have genomes with an unconventional gene organization as compared to eukaryotic genomes. Splicing and multiple open reading frames encoded by a single mRNA are common in viruses, suggesting that in eukaryotic cells, some virus mRNAs could be targeted by NMD because of the presence of “PTCs”. In keeping with this view, down-regulation of NMD factors results in a strong increase in the production of viral proteins (140). Viruses have developed strategies for escaping NMD and using components of the NMD machinery to their own advantage. HIV-1, for instance, uses UPF1 and other NMD factors to facilitate the export of its RNA molecules from the nucleus thanks to a Rev-UPF1 interaction that prevents UPF2 from binding UPF1 (141). Once in the cytoplasm, UPF1 remains bound to the viral RNA and can also be encapsidated in virions (142). Down-regulation of UPF1 reduces the infectivity of HIV virions, suggesting an unexpected role of UPF1 in promoting infectivity. This could provide a basis for an interesting antiviral approach.


NMD has been found in all eukaryotic organisms studied to date. The role of this quality control mechanism is to eliminate faulty mRNAs harboring a PTC in order to prevent the synthesis of aberrant truncated proteins (1, 2, 8). In eukaryotes, NMD is also a regulatory pathway for some wild-type genes: 5–10% of human genes, for instance (118, 143). NMD occurs in the cytoplasm, during or after mRNA export to this compartment and before steady-state translation (144). Some proteins involved in NMD, however, are loaded onto the mRNA in the nucleus (63, 71, 145). To accurately perform its role, NMD must be activated at the right moment, at the right place, and on the right mRNA. All these parameters can be influenced by cell conditions or stimuli, and various other signaling and metabolic pathways are involved. Exactly how many and which mechanisms affect NMD activation is still under investigation, and the list presented here is far from exhaustive. We would like to apologize to colleagues whose work is not cited in this review because of space limitation. As described here, many different mechanisms are also tightly linked to NMD, such as the miRNA gene regulation pathway, the unfolded protein response, and apoptosis. The interaction of all these pathways with NMD reflects the central role of NMD in cell homeostasis and in the development of multi-cellular organisms. We can divide the interactions between these processes and NMD into three categories: (i) bidirectional interactions in which NMD regulates a mechanism and this mechanism can also influence NMD efficiency; (ii) unidirectional interactions in which NMD influences a specific process; (iii) unidirectional interactions in which a process influences NMD (Fig. 2). It is crucial to study and understand these interactions, especially because inhibiting NMD appears as an attractive therapeutic approach to correcting nonsense mutations in genetic diseases (6, 146). Such therapies will have to allow some NMD to occur or they will have to target their NMD-inhibiting action to specific nonsense-mutation-containing mRNAs rather than to all PTC-containing mRNAs.

Fig. 1. Models of NMD activation. (A) The EJC-dependent model. PTCs are recognized if they are present more than 50–55 nucleotides upstream of an exon-exon junction during the pioneer round of translation. The mRNA carries the CBP20/CBP80 hetero-dimer at the 5′cap and PABPN1 and PABPC1 on the poly(A) tail at the 3′ end. In this model UPF1 is recruited by the UPF3X and UPF2 proteins bound to the exon junction complex (EJC). Translation termination occurs because UPF1 interacts with the release factors eRF1 () and eRF3 (). (B) The PTC-PABPC1 distance model. PTC recognition is based on the probability that translation termination will occur via the interaction between UPF1 and release factors (NMD translation termination) or via the interaction between PABPC1 and release factors (normal translation termination).
Fig. 2. NMD interacts with cellular processes in different ways: (i) bidirectional interactions in which NMD and a cellular process influence each other (bidirectional arrows); (ii) unidirectional interactions in which NMD influences a cellular process (arrows pointing a cellular process); (iii) unidirectional interactions in which a cellular process regulates NMD efficiency (arrows pointing NMD).

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