BMB Reports 2017; 50(3): 132-137  
Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells
Sunhwa Oh1, Hyungjoo Kim1, KeeSoo Nam1 & Incheol Shin1,2,*
1Department of Life Science, 2Natural Science Institute, Hanyang University, Seoul 04763, Korea
Correspondence to: Tel: +82-2-2220-2562; Fax: +82-2-2298-2562; E-mail: incheol@hanyang.ac.kr
Received: November 11, 2016; Published online: March 31, 2017.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Abstract
Elevated glucose levels in cancer cells can be attributed to increased levels of glucose transporter (GLUT) proteins. Glut1 expression is increased in human malignant cells. To investigate alternative roles of Glut1 in breast cancer, we silenced Glut1 in triple-negative breast-cancer cell lines using a short hairpin RNA (shRNA) system. Glut1 silencing was verified by Western blotting and qRT-PCR. Knockdown of Glut1 resulted in decreased cell proliferation, glucose uptake, migration, and invasion through modulation of the EGFR/ MAPK signaling pathway and integrin β1/Src/FAK signaling pathways. These results suggest that Glut1 not only plays a role as a glucose transporter, but also acts as a regulator of signaling cascades in the tumorigenesis of breast cancer.
Keywords: Breast cancer cell, EGFR, Glut, Integrin β1, Triple-negative breast cancer


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