
Polymer brush is a soft material unit tethered covalently on the surface of scaffolds. It can induce functional and structural modification of a substrate’s properties. Such surface coating approach has attracted special attentions in the fields of stem cell biology, tissue engineering, and regenerative medicine due to facile fabrication, usability of various polymers, extracellular matrix (ECM)-like structural features, and
Polymer brush is a soft material unit with an entangled structure that is covalently tethered on the surface of scaffolds or substrates (1, 2). Polymer brush can assign and tailor diverse structural and functional features of polymers on the scaffold or support surface. Its easy fabrication has made it applicable in various fields such as electronics, sensors, anti-fouling, catalysis, purification, and energy (1, 2).
There have been recent advancements in the coating process of polymer brush for stem cell biology, tissue engineering, and regenerative medicine (1, 3). This is due to the facile attachment of bioactive materials to polymer brush which can stimulate cells to control in specific biological directions, diverse scaffold materials for polymer brush coating, and simple fabrication and conjugation process. In addition, various functional polymers can be used in almost unlimited ways, and polymer chain length, density, and microstructures of polymer brush can be easily adjusted. It can not only mimic extracellular matrix (ECM)-like structures to induce cell adhesion and growth but it has been reported to be highly stable
For biomedical implant to be successively developed clinically, biocompatibility of implant materials need to be outstanding. Cell adhesion and proliferation also need to be active so that it can be incorporated onto the host while having good inflammatory resistance and smooth tissue reorganization (4, 5). For this purpose, research is being actively conducted to constitute and control the microenvironments on scaffold surface using polymer brush to enhance cell compatible properties and modulate stem cell differentiation on its tailored surface.
This review summarizes recently reported studies on polymer brush coating for the regulation of cell behaviors for bioapplications in tissue engineering and regenerative medicine and cellular responses toward microstructures stemmed from precisely implemented nanometer-sized polymer brush. Recent research in stem cell engineering using polymer brush will be introduced along with studies based on its application in implants and three dimensional (3D) structures with the polymer brush. The effects of microstructures formed by polymer brush in such a microenvironment and materials that result in complex effects on cells will also be reviewed.
In order to effectively apply polymer brush in tissue engineering and regenerative medicine, a prior understanding is needed for the procedure of polymer brush fabrication, its physico-chemical features, and the analytic processes for characterizing them. Therefore, the properties of polymer brush and its analysis methodologies are also described by focusing on the factors that can affect cell behaviors for its application in medical implants.
There are two major approaches in tailoring the physicochemical properties of an interface by attaching various molecular constituents on the scaffold/substrate: polymer brush approach and self-assembled monolayer (SAM) approach. Both processes have been intensively applied in cell biological research because these approaches not only give a new function to the surface of substrates by attaching diverse molecules, but also result in tailored interfaces similar to an ECM’s microstructure that plays important role in cellular adhesion, formation, and proliferation (4, 6).
SAM was developed by George Whitesides group. It describes molecular assemblies, exquisitely arranged organic molecules that spontaneously form on a substrate’s surface in a single layer. Due to the self-reorganizing property of these organic elements, they can be coated meticulously. For example, alkanethiols and its assortatively designed patterns via microcontact printing and dip-pen nanolithography on gold thin films of silica glass are widely used in cell research. Regardless of the advantages of SAM, limited types of useable organic molecules, unconfirmed stability on other substrates than gold, low
The polymer brush approach covalently tethers polymer chains on the surface of chemically reactive substrates. It has attracted special attention due to its facile fabrication process, compatibility with various scaffold materials such as glass, silicon, gold, silver, and titanium, flexibility in adjusting polymer chain length and density, and the possibility to conjugate additional functional molecules. Polymer brush approach can be divided as grafting-to and grafting-from techniques depending on the fabrication process. The grafting-to technique covalently anchors the polymer chain or polymer unit onto the reactive surface of the scaffold while the grafting-from technique forms a polymer chain or polymer unit via polymerization from polymeric initiating moiety on the scaffold surface. The grafting-to technique forms a looser brush density than the grafting-from technique because of steric hindrance caused by the previously attached polymers in its coupling process at the interface (Fig. 1A, B). According to its fabrication approaches and related parameters, polymer chain length is adjustable (2). Various attributes of polymer brush compared to SAM approach are summarized in Table 1.
For implants to be successfully integrated into the host system, cell adhesion, formation, proliferation, and differentiation have to occur actively on the scaffold-polymer brush interface. The polymer brush on a scaffold that can influence such cell behaviors needs to be quantitatively analyzed. Hydrophobicity, roughness, stiffness/elasticity, and chemical composition are important factors that can affect cell behaviors and manipulation. Generally, as the surface hydrophobicity rises, the rate of cell attachment and spreading will drop. However, as can be seen in polyethylene glycol (PEG) and poly (sulfobetaine methacrylate) (polySBMA), the hydrophilicity of polymer brush does not always result in improved cell adhesion (7, 9, 10). Surface hyrophobicity and hydrophilicity can be measured by the wettability via the increase and decrease in surface tension of liquid drops formed on the substrate surface. This can be quantified by measuring the static contact angle. Whereas angle measured from the surface rises in droplets on hydrophobic surfaces, it drops on hydrophilic surfaces because the droplets spread flat (11).
Roughness, a topological property of a surface, plays an important role in cell adhesion, morphogenesis, and proliferation, especially in implant and tissue formation (7). Scanning electron microscopy (SEM) is usually used to observe the overall surface morphology. Atomic force microscopy (AFM) is normally used to quantitatively measure surface roughness more elaborately. Roughness via AFM is quantitatively represented by taking the root mean square of the difference between the individual peaks and the average height within specific lines or areas (12, 13).
Rigidity/elasticity, also referred to as stiffness/softness, of the mechanical property of a substrate is one influential parameter that can affect cell attachment, growth, and differentiation (7). Tilghman
Chemical compositions of a surface need to be analyzed to determine if polymers or biopolymers are appropriately attached to the surface. Infrared (IR) and Raman spectrometry can be used to identify the substances of the functional groups of chemicals/polymers. X-ray photoelectron spectroscopy (XPS) can be used to analyze the electronic state and element composition of the surface. Thermal gravimetric analysis (TGA) can be utilized to detect mass reduction by a rise in temperature, ultimately monitoring the unique phase transition caused by vaporization, sublimation, and adsorption according to its own physicochemical properties.
In addition, ellipsometry and AFM can be used to measure the length of a folded polymer chain (height from bare scaffold). Cyclic voltammetry (CV) or surface zeta potential can be used to determine the electrochemical properties of a substance. Fluorescence image analysis via conjugation of fluorescent materials is obtained to identify the overall coverage of the target molecules. All these parameters and apparatuses for investigating surface properties are summarized in Table 2, focusing on factors that can influence cell behaviors.
These tools for analysis enable the micro-architecture and physico-chemical properties of a polymer brush to be characterized precisely, which can directly correlate its features with cell behaviors to aid the designing of basic coating platform using polymer brushes in tissue engineering and regenerative medicine.
Polymer brush has been widely used for protein adsorption, biosensing, anti-fouling, cell culture, and regulation of cell behavior in the field of biomedicine. Cellular responses toward bioactive polymer brush and its control on cell behaviors have been especially studied in tissue engineering. Cellular manipulation using thermoresponsive polymer brush is also being actively studied.
Currently, studies on polymer brush have expanded from regulating stem cell behaviors to 3D support/implant coating for its application in regenerative medicine and clinical biomedicine. The delicately designed micro-architectures of polymer brush of nanometer-sized chain length and the resulting cellular responses to both polymer and scaffold materials are being systematically investigated (Fig. 1C). Such studies on polymer brush for tissue engineering will collectively contribute to the design of the most optimal platform for clinical implants.
Stem cell studies on polymer brush have been directed toward regulating stem cell behaviors by using polymer brush itself or bioactive properties of additionally attached biopolymers (18, 21). Poly[2(methacryloyloxy)ethyl dimethyl-(3-ulfopropyl) ammonium hydroxide] (PMEDSAH) polymer brush has been shown to be able to maintain undifferentiated human embryonic stem cell (hESC) in a long-term culture (19). Poly(OEGMA-
Kumar
Gautrot and Connelly
Most medical implants have three-dimensional structures. Research in polymer brush fabrication of 3D scaffold is actively underway to study cell adhesion and tissue formation on such 3D support. Gunnewiek
Hydrogel-based support is a 3D architecture/scaffold that is in the limelight because of its formation of artificial ECM network. Its fabrication methodology of bioresponsive brush by conjugating biopolymers on a hydrogel scaffold is being researched actively. It was reported that the bioactivity of a retinal precursor cell (RPC) was tested on an agarose hydrogel that patternized the protein brush of sonic hedgehog (SHH) and ciliary neurotrophic factor (CNTF) in 3D hydrogel using a two photon irradiation method (35). Patternized RGD in poly(ethylene glycol) (PEG) - diacrylate (PEGDA) 3D hydrogel using two photon absorption lithography can affect cell confinement and migration (36). PEG-based hydrogel simultaneously coupled with integrin-mimicked peptide and matrix metalloproteinase (MMP) substrate has been fabricated to study cell invasion for tissue regeneration or bone regeneration (37, 38).
Besides stem cell engineering on polymer brush and polymer brush coating for 3D support, the delicate modulation of the thickness and density of a polymer brush is also an important factor that can affect cell behaviors due to resulting changes in its micro-architectures and physico-chemical characteristics. A fabrication process for gradient polymer brush grafting has been developed to control its chain length and density (43–45). This process can result in various degrees of protein adsorption and cell adhesion according to the gradation of polymer brushes (39). Along with polymer chain length, the microstructure of poly[{2-(methacryloyloxy) ethyl} dimethyl-(3-sulfopropyl) ammonium hydroxide] (PMEDSAH) brush can change. Simulation results have shown that well-modulated thickness of polymer brush can affect its changes in micro-architecture and transition of physical properties such as wettability which make it possible to design the most optimal polymer brush for stem cell culture (30). A simulation of nuclear pore complex (NPC) based on polymer brush model has shown that NPC proteins placed in the different sections of the nuclear pore can form various disordered polypeptides as a conserved functional feature, and formation of the unique biopolymer brush architecture can play an important role in regulating the open-close of the nuclear pore to transport biological cargos (40).
Interestingly, as the polymer chain is shortened within a few nanometers, the microstructure and its chemical features also changed. Then cells not only interact with polymer brush, but also with scaffolds, the base materials. Gon
Chiang
Polymer brush is the most optimal scaffold surface grafting approach for biomedical implant, regenerative medicine, and tissue engineering. It is supported by current intensive researches on the use of polymer brush in stem cell engineering and polymer brush coating for 3D support in tissue engineering. The types of polymer used in polymer brush, polymer chain length, and brush density can modulate the microstructures and physico-chemical properties of polymer brush. It can lead to derive the most optimal cultivation condition for stem cell engineering. Furthermore, shortening polymer chain length by a few nanometers to induce simultaneous interaction between polymer and scaffold materials with cells can be used to predict the effects of low density brush, heterogeneous coating, and flaw during grafting on cellular response and tissue formation. As a result, the planning and fabrication of various micro-architecture of polymer brush, precise analysis and modulation of its physico-chemical properties, and systematic studies on its cellular responses will contribute to the optimal design of polymer brush for biomedical implant application.
We thank Mr. Gyoung Chan Seo for helping us with the figure. We also thank Dr. Soosang Chae for his valuable suggestions for the manuscript. This work (C0395024) was supported by Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration in 2016. It was also supported by Hannam University Research Fund.
Comparison of the physico-chemical properties between polymer brush and self-assembled monolayer (SAM)
Polymer brush | SAM (self-assembled monolayer) | ||
---|---|---|---|
Grafting-to | Grafting-from | ||
Grafting molecule | Almost all types of polymers | Mainly alkanethiol & alkyl silnae | |
Micro-architecture | Various and complex polymeric structures | Well assembled molecular monolayer | |
Scaffold materials | Glass, titanium, gold, silver, silicon, etc | Gold thin film, oxide-formed substrate | |
Thickness | High tenability by adjusting polymer chain length | Thin: one molecular layer | |
Coating defects | Presence: short polymer chain | Presence of defects and pinhole | |
High stability | Low stability | ||
Coating density | Loosely packed | Densely packed | Densely packed |
Fabrication approach | Various chemical coupling between polymer and surface | Various polymerization on the surface | Thiol-gold bond & silane linkage |
Characterization of the physico-chemical properties of polymer brushes on scaffold focusing on factors that can affect cell behaviors
Wettability | Roughness | Rigidity/elasticity | Chemical composition | Height | |
---|---|---|---|---|---|
Tools for analysis | Contact angle measurement | AFM, SEM | AFM (force mode) | IR & Raman spectrometery, XPS, TGA | Ellipsometry, AFM |
Parameters | Angle between surface of a liquid drop and substrate | Root mean square of the height of surface contour | The degree of deflection of AFM cantilever at contact point | Chemical functional group, element composition, thermal decomposition | Height from the surface of scaffold |
Properties | Hydrophobicity & hydrophilicity | Topology & geometry | Mechanical strength | Presence of target polymers on the surface | Thickness of polymer brush |