BMB Rep. 2016; 49(10): 554-559  
Mycobacterium abscessus D-alanyl-D-alanine dipeptidase induces the maturation of dendritic cells and promotes Th1-biased immunity
Seung Jun Lee1,#, Jong-Hwa Jang2,#, Gun Young Yoon1, Da Rae Kang1, Hee Jo Park1, Sung Jae Shin3, Hee Dong Han1, Tae Heung Kang1, Won Sun Park4, Young Kyung Yoon5, Byoung Yul Soh6, In Duk Jung1,* & Yeong-Min Park1,*

1Department of Immunology, Laboratory of Dendritic Cell Differentiation and Regulation, School of Medicine, Konkuk University, Chungju 27478, 2Department of Dental Hygiene, Hanseo University, Seosan 31962, 3Department of Microbiology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, 4Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 24341, 5Division of Infectious Diseases, Department of Internal Medicine, Korea University Anam Hospital, College of Medicine, Korea University, Seoul 02841, 6Department of Biochemistry, College of Medicine, Seonam University, Namwon 55724, Korea

Correspondence to: In Duk Jung, Tel: +82-2-2049-6330; Fax: +82-2-2049-6192; E-mail: jungid@kku.ac.kr, Yeong-Min Park, Tel: +82-2-2049-6330; Fax: +82-2-2049-6192; E-mail: immun3023@kku.ac.kr
#These authors contributed equally to this work.
Received: May 10, 2016; Revised: May 30, 2016; Accepted: July 19, 2016; Published online: October 31, 2016.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Abstract
Mycobacterium abscessus, a member of the group of non-tuberculous mycobacteria, has been identified as an emerging pulmonary pathogen in humans. However, little is known about the protective immune response of antigenpresenting cells, such as dendritic cells (DCs), which guard against M. abscessus infection. The M. abscessus gene MAB1843 encodes D-alanyl-D-alanine dipeptidase, which catalyzes the hydrolysis of D-alanyl-D-alanine dipeptide. We investigated whether MAB1843 is able to interact with DCs to enhance the effectiveness of the host's immune response. MAB1843 was found to induce DC maturation via toll-like receptor 4 and its downstream signaling pathways, such as the mitogen-activated protein kinase and nuclear factor kappa B pathways. In addition, MAB1843-treated DCs stimulated the proliferation of T cells and promoted Th1 polarization. Our results indicate that MAB1843 could potentially regulate the immune response to M. abscessus, making it important in the development of an effective vaccine against this mycobacterium.
Keywords: Dendritic cells, MAB1843, MAPK, Mycobacterium abscessus, Th1 polarization


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